RGD Reference Report - Renal expression of the ammonia transporters, Rhbg and Rhcg, in response to chronic metabolic acidosis. - Rat Genome Database

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Renal expression of the ammonia transporters, Rhbg and Rhcg, in response to chronic metabolic acidosis.

Authors: Seshadri, RM  Klein, JD  Kozlowski, S  Sands, JM  Kim, YH  Han, KH  Handlogten, ME  Verlander, JW  Weiner, ID 
Citation: Seshadri RM, etal., Am J Physiol Renal Physiol. 2006 Feb;290(2):F397-408. Epub 2005 Sep 6.
RGD ID: 8554685
Pubmed: PMID:16144966   (View Abstract at PubMed)
DOI: DOI:10.1152/ajprenal.00162.2005   (Journal Full-text)

Chronic metabolic acidosis induces dramatic increases in net acid excretion that are predominantly due to increases in urinary ammonia excretion. The current study examines whether this increase is associated with changes in the expression of the renal ammonia transporter family members, Rh B glycoprotein (Rhbg) and Rh C glycoprotein (Rhcg). Chronic metabolic acidosis was induced in Sprague-Dawley rats by HCl ingestion for 1 wk; control animals were pair-fed. After 1 wk, metabolic acidosis had developed, and urinary ammonia excretion increased significantly. Rhcg protein expression was increased in both the outer medulla and the base of the inner medulla. Intercalated cells in the outer medullary collecting duct (OMCD) and in the inner medullary collecting duct (IMCD) in acid-loaded animals protruded into the tubule lumen and had a sharp, discrete band of apical Rhcg immunoreactivity, compared with a flatter cell profile and a broad band of apical immunolabel in control kidneys. In addition, basolateral Rhcg immunoreactivity was observed in both control and acidotic kidneys. Cortical Rhcg protein expression and immunoreactivity were not detectably altered. Rhcg mRNA expression was not significantly altered in the cortex, outer medulla, or inner medulla by chronic metabolic acidosis. Rhbg protein and mRNA expression were unchanged in the cortex, outer and inner medulla, and no changes in Rhbg immunolabel were evident in these regions. We conclude that chronic metabolic acidosis increases Rhcg protein expression in intercalated cells in the OMCD and in the IMCD, where it is likely to mediate an important role in the increased urinary ammonia excretion.

RGD Manual Disease Annotations    Click to see Annotation Detail View
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
metabolic acidosis  ISORhcg (Rattus norvegicus)8554685; 8554685protein:increased expression:medulla and kidney collecting duct intercalated cellRGD 
metabolic acidosis  IEP 8554685protein:increased expression:medulla and kidney collecting duct intercalated cellRGD 

Gene Ontology Annotations    Click to see Annotation Detail View

Cellular Component
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
apical plasma membrane located_inIDA 8554685PMID:16144966UniProt 
basolateral plasma membrane located_inIDA 8554685; 8554685; 8554685PMID:16144966UniProt 

Objects Annotated

Genes (Rattus norvegicus)
Rhbg  (Rh family B glycoprotein)
Rhcg  (Rh family, C glycoprotein)
Slc4a1  (solute carrier family 4 member 1 (Diego blood group))

Genes (Mus musculus)
Rhcg  (Rhesus blood group-associated C glycoprotein)

Genes (Homo sapiens)
RHCG  (Rh family C glycoprotein)


Additional Information