RGD Reference Report - Clustering of Shaker-type K+ channels by interaction with a family of membrane-associated guanylate kinases. - Rat Genome Database

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Clustering of Shaker-type K+ channels by interaction with a family of membrane-associated guanylate kinases.

Authors: Kim, E  Niethammer, M  Rothschild, A  Jan, YN  Sheng, M 
Citation: Kim E, etal., Nature. 1995 Nov 2;378(6552):85-8.
RGD ID: 8554588
Pubmed: PMID:7477295   (View Abstract at PubMed)
DOI: DOI:10.1038/378085a0   (Journal Full-text)

ANCHORING of ion channels at specific subcellular sites is critical for neuronal signalling, but the mechanisms underlying channel localization and clustering are largely unknown (reviewed in ref. 1). Voltage-gated K+ channels are concentrated in various neuronal domains, including presynaptic terminals, nodes of Ranvier and dendrites, where they regulate local membrane excitability. Here we present functional and biochemical evidence that cell-surface clustering of Shaker-subfamily K+ channels is mediated by the PSD-95 family of membrane-associated putative guanylate kinases, as a result of direct binding of the carboxy-terminal cytoplasmic tails to the K+ channel subunits to two PDZ (also known as GLGF or DHR) domains in the PSD-95 protein. The ability of PDZ domains to function as independent modules for protein-protein interaction, and their presence in other junction-associated molecules (such as ZO-1 (ref. 3) and syntrophin), suggest that PDZ-domain-containing polypeptides may be widely involved in the organization of proteins at sites of membrane specialization.



Gene Ontology Annotations    Click to see Annotation Detail View

Molecular Function

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Kcna1Ratprotein binding enablesIPIUniProtKB:P78352PMID:7477295IntAct 
Kcna2Ratprotein binding enablesIPIUniProtKB:P78352PMID:7477295IntAct 
Kcna3Ratprotein binding enablesIPIUniProtKB:P78352PMID:7477295IntAct 
Kcna4Ratprotein binding enablesIPIUniProtKB:P78352PMID:7477295IntAct 

Objects Annotated

Genes (Rattus norvegicus)
Kcna1  (potassium voltage-gated channel subfamily A member 1)
Kcna2  (potassium voltage-gated channel subfamily A member 2)
Kcna3  (potassium voltage-gated channel subfamily A member 3)
Kcna4  (potassium voltage-gated channel subfamily A member 4)


Additional Information