RGD Reference Report - Interaction between SAP97 and PSD-95, two Maguk proteins involved in synaptic trafficking of AMPA receptors. - Rat Genome Database

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Interaction between SAP97 and PSD-95, two Maguk proteins involved in synaptic trafficking of AMPA receptors.

Authors: Cai, C  Li, H  Rivera, C  Keinanen, K 
Citation: Cai C, etal., J Biol Chem. 2006 Feb 17;281(7):4267-73. Epub 2005 Dec 6.
RGD ID: 8554542
Pubmed: PMID:16332687   (View Abstract at PubMed)
DOI: DOI:10.1074/jbc.M505886200   (Journal Full-text)

Synapse-associated protein 97 (SAP97) and postsynaptic density 95 (PSD-95) are closely related membrane-associated guanylate kinase homologs (Maguks) implicated in the synaptic targeting and anchoring of alpha-amino-5-methyl-3-hydroxy-4-isoxazolepropionic acid (AMPA)-selective glutamate receptors. Prompted by accumulating evidence for an oligomeric nature of Maguks, we examined the potential of SAP97 and PSD-95 to form heteromeric complexes. SAP97 and PSD-95 coimmunoprecipitated from rat brain detergent extracts and subsequent glutathione S-transferase pull-down and immunoprecipitation experiments showed that the interaction is mediated by binding of the N-terminal segment of SAP97 (SAP97(NTD)) to the Src homology 3 domain of PSD-95 (PSD-95(SH3)). In cultured hippocampal neurons, expression of green fluorescent protein-tagged PSD-95 triggered accumulation of SAP97 in synaptic spines, which was totally inhibited by coexpression of PSD-95(SH3). Furthermore, overexpression of green fluorescent protein-PSD-95 induced dendritic clustering of GluR-A subunit-containing AMPA receptors, which was strongly inhibited by cotransfection with SAP97(NTD) and PSD-95(SH3) constructs. Our results demonstrated a direct interaction between SAP97 and PSD-95 and suggested that this association may play a functional role in the trafficking and clustering of AMPA receptors.

Gene Ontology Annotations    Click to see Annotation Detail View

Molecular Function
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
protein binding enablesIPIUniProtKB:P310168554542PMID:16332687IntAct 
protein binding enablesIPIUniProtKB:Q12959-28554542PMID:16332687IntAct 
protein binding enablesIPIUniProtKB:Q626968554542PMID:16332687IntAct 

Objects Annotated

Genes (Rattus norvegicus)
Dlg1  (discs large MAGUK scaffold protein 1)
Dlg4  (discs large MAGUK scaffold protein 4)


Additional Information