RGD Reference Report - Ghrelin is present in pancreatic alpha-cells of humans and rats and stimulates insulin secretion. - Rat Genome Database
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Ghrelin is present in pancreatic alpha-cells of humans and rats and stimulates insulin secretion.

Authors: Date, Y  Nakazato, M  Hashiguchi, S  Dezaki, K  Mondal, MS  Hosoda, H  Kojima, M  Kangawa, K  Arima, T  Matsuo, H  Yada, T  Matsukura, S 
Citation: Date Y, etal., Diabetes. 2002 Jan;51(1):124-9.
RGD ID: 8554453
Pubmed: (View Article at PubMed) PMID:11756331

Ghrelin, a novel growth hormone-releasing peptide isolated from human and rat stomach, is a 28-amino acid peptide with a posttranslational acylation modification that is indispensable for stimulating growth hormone secretion by increasing intracellular Ca(2+) concentration. It also functions in the regulation of feeding behavior, energy metabolism, and gastric acid secretion and motility. Using two different antibodies against the NH(2)- and COOH-terminal regions of ghrelin, we studied its localization in human and rat pancreas by immunohistochemistry. Ghrelin-immunoreactive cells were identified at the periphery of pancreatic islets in both species. Ghrelin co-localized exclusively with glucagon in rat islets, indicating that it is produced in alpha-cells. We identified ghrelin and des-acyl ghrelin in the rat pancreas using reverse-phase high-performance liquid chromatography combined with two radioimmunoassays. We also detected mRNA encoding ghrelin and its receptor in the rat pancreatic islets. Ghrelin increased the cytosolic free Ca(2+) concentration in beta-cells and stimulated insulin secretion when it was added to isolated rat pancreatic islets. These findings indicate that ghrelin may regulate islet function in an endocrine and/or paracrine manner.

Annotation

Gene Ontology Annotations    

Biological Process

Objects Annotated

Genes (Rattus norvegicus)
Ghrl  (ghrelin and obestatin prepropeptide)


Additional Information