RGD Reference Report - S100A1 is a novel molecular chaperone and a member of the Hsp70/Hsp90 multichaperone complex. - Rat Genome Database

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S100A1 is a novel molecular chaperone and a member of the Hsp70/Hsp90 multichaperone complex.

Authors: Okada, M  Hatakeyama, T  Itoh, H  Tokuta, N  Tokumitsu, H  Kobayashi, R 
Citation: Okada M, etal., J Biol Chem. 2004 Feb 6;279(6):4221-33. Epub 2003 Nov 24.
RGD ID: 8554444
Pubmed: PMID:14638689   (View Abstract at PubMed)
DOI: DOI:10.1074/jbc.M309014200   (Journal Full-text)

Although calmodulin is known to be a component of the Hsp70/Hsp90 multichaperone complex, the functional role of the protein remains uncertain. In this study, we have identified S100A1, but not calmodulin or other S100 proteins, as a potent molecular chaperone and a new member of the multichaperone complex. Glutathione S-transferase pull-down assays and co-immunoprecipitation experiments indicated the formation of stable complexes between S100A1 and Hsp90, Hsp70, FKBP52, and CyP40 both in vitro and in mammalian cells. S100A1 potently protected citrate synthase, aldolase, glyceraldehyde-3-phosphate dehydrogenase, and rhodanese from heat-induced aggregation and suppressed the aggregation of chemically denatured rhodanese and citrate synthase during the refolding pathway. In addition, S100A1 suppressed the heat-induced inactivation of citrate synthase activity, similar to that for Hsp90 and p23. The chaperone activity of S100A1 was antagonized by calmodulin antagonists, such as fluphenazine and prenylamine, that is, indeed an intrinsic function of the protein. The overexpression of S100A1 in COS-7 cells protected transiently expressed firefly luciferase and Escherichia coli beta-galactosidase from inactivation during heat shock. The results demonstrate a novel physiological function for S100A1 and bring us closer to a comprehensive understanding of the molecular mechanisms of the Hsp70/Hsp90 multichaperone complex.



Gene Ontology Annotations    Click to see Annotation Detail View

Molecular Function

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
S100a1Ratprotein binding enablesIPIUniProtKB:P07900PMID:14638689IntAct 
S100a1Ratprotein binding enablesIPIUniProtKB:P26882PMID:14638689IntAct 
S100a1Ratprotein binding enablesIPIUniProtKB:Q02790PMID:14638689IntAct 

Objects Annotated

Genes (Rattus norvegicus)
S100a1  (S100 calcium binding protein A1)


Additional Information