RGD Reference Report - Marlin-1 and conventional kinesin link GABAB receptors to the cytoskeleton and regulate receptor transport. - Rat Genome Database

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Marlin-1 and conventional kinesin link GABAB receptors to the cytoskeleton and regulate receptor transport.

Authors: Vidal, RL  Ramirez, OA  Sandoval, L  Koenig-Robert, R  Hartel, S  Couve, A 
Citation: Vidal RL, etal., Mol Cell Neurosci. 2007 Jul;35(3):501-12. Epub 2007 May 1.
RGD ID: 8554443
Pubmed: PMID:17532644   (View Abstract at PubMed)
DOI: DOI:10.1016/j.mcn.2007.04.008   (Journal Full-text)

The cytoskeleton and cytoskeletal motors play a fundamental role in neurotransmitter receptor trafficking, but proteins that link GABA(B) receptors (GABA(B)Rs) to the cytoskeleton have not been described. We recently identified Marlin-1, a protein that interacts with GABA(B)R1. Here, we explore the association of GABA(B)Rs and Marlin-1 to the cytoskeleton using a combination of biochemistry, microscopy and live cell imaging. Our results indicate that Marlin-1 is associated to microtubules and the molecular motor kinesin-I. We demonstrate that a fraction of Marlin-1 is mobile in dendrites of cultured hippocampal neurons and that mobility is microtubule-dependent. We also show that GABA(B)Rs interact robustly with kinesin-I and that intracellular membranes containing GABA(B)Rs are sensitive to treatments that disrupt a protein complex containing Marlin-1, kinesin-I and tubulin. Finally, we report that a kinesin-I mutant severely impairs receptor transport. We conclude that Marlin-1 and kinesin-1 link GABA(B)Rs to the tubulin cytoskeleton in neurons.

Gene Ontology Annotations    Click to see Annotation Detail View

Cellular Component
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
microtubule cytoskeleton located_inIDA 8554443PMID:17532644MGI 

Objects Annotated

Genes (Rattus norvegicus)
Jakmip1  (janus kinase and microtubule interacting protein 1)


Additional Information