RGD Reference Report - Interaction of the DYNLT (TCTEX1/RP3) light chains and the intermediate chains reveals novel intersubunit regulation during assembly of the dynein complex.

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Interaction of the DYNLT (TCTEX1/RP3) light chains and the intermediate chains reveals novel intersubunit regulation during assembly of the dynein complex.
Authors:	Lo, KW Kogoy, JM Rasoul, BA King, SM Pfister, KK
Citation:	Lo KW, etal., J Biol Chem. 2007 Dec 21;282(51):36871-8. Epub 2007 Oct 27.
RGD ID:	8554399
Pubmed:	PMID:17965411 (View Abstract at PubMed)
DOI:	DOI:10.1074/jbc.M705991200 (Journal Full-text)
The cytoplasmic dynein 1 cargo binding domain is formed by five subunits including the intermediate chain and the DYNLT, DYNLL, and DYNLRB light chain families. Six isoforms of the intermediate chain and two isoforms of each of the light chain families have been identified in mammals. There is evidence that different subunit isoforms are involved in regulating dynein function, in particular linking dynein to different cargoes. However, it is unclear how the subunit isoforms are assembled or if there is any specificity to their interactions. Co-immunoprecipitation using DYNLT-specific antibodies reveals that dynein complexes with DYNLT light chains also contain the DYNLL and DYNLRB light chains. The DYNLT light chains, but not DYNLL light chains, associate exclusively with the dynein complex. Yeast two-hybrid and co-immunoprecipitation assays demonstrate that both members of the DYNLT family are capable of forming homodimers and heterodimers. In addition, both homodimers of the DYNLT family bind all six intermediate chain isoforms. However, DYNLT heterodimers do not bind to the intermediate chain. Thus, whereas all combinations of DYNLT light chain dimers can be made, not all of the possible combinations of the isoforms are utilized during the assembly of the dynein complex.

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Cellular Component

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Object Symbol	Species	Term	Qualifier	Evidence	With	Notes	Source	Original Reference(s)
Dynll1	Rat	cytoplasmic dynein complex		IDA			RGD
Dynll2	Rat	cytoplasmic dynein complex		IDA			RGD

Molecular Function

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Object Symbol	Species	Term	Qualifier	Evidence	With	Notes	Source	Original Reference(s)
Dynll1	Rat	identical protein binding		IPI	Dynll1 (Rattus norvegicus)	homodimerization	RGD
Dynll2	Rat	identical protein binding		IPI	Dynll2 (Rattus norvegicus)	homodimerization	RGD
Dynll1	Rat	protein-containing complex binding		IDA		heterodimerization	RGD
Dynll2	Rat	protein-containing complex binding		IDA		heterodimerization	RGD

Objects Annotated

Genes (Rattus norvegicus)

Dynll1 (dynein light chain LC8-type 1)

Dynll2 (dynein light chain LC8-type 2)

Objects referenced in this article

Gene	Dynlt3	dynein light chain Tctex-type 3	Rattus norvegicus

Additional Information

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Interaction of the DYNLT (TCTEX1/RP3) light chains and the intermediate chains reveals novel intersubunit regulation during assembly of the dynein complex.