RGD Reference Report - Conformational changes in the AAA ATPase p97-p47 adaptor complex. - Rat Genome Database

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Conformational changes in the AAA ATPase p97-p47 adaptor complex.

Authors: Beuron, F  Dreveny, I  Yuan, X  Pye, VE  McKeown, C  Briggs, LC  Cliff, MJ  Kaneko, Y  Wallis, R  Isaacson, RL  Ladbury, JE  Matthews, SJ  Kondo, H  Zhang, X  Freemont, PS 
Citation: Beuron F, etal., EMBO J. 2006 May 3;25(9):1967-76. Epub 2006 Apr 6.
RGD ID: 8554186
Pubmed: PMID:16601695   (View Abstract at PubMed)
PMCID: PMC1456939   (View Article at PubMed Central)
DOI: DOI:10.1038/sj.emboj.7601055   (Journal Full-text)

The AAA+ATPase p97/VCP, helped by adaptor proteins, exerts its essential role in cellular events such as endoplasmic reticulum-associated protein degradation or the reassembly of Golgi, ER and the nuclear envelope after mitosis. Here, we report the three-dimensional cryo-electron microscopy structures at approximately 20 Angstroms resolution in two nucleotide states of the endogenous hexameric p97 in complex with a recombinant p47 trimer, one of the major p97 adaptor proteins involved in membrane fusion. Depending on the nucleotide state, we observe the p47 trimer to be in two distinct arrangements on top of the p97 hexamer. By combining the EM data with NMR and other biophysical measurements, we propose a model of ATP-dependent p97(N) domain motions that lead to a rearrangement of p47 domains, which could result in the disassembly of target protein complexes.

Gene Ontology Annotations    

Cellular Component

Molecular Function

Objects Annotated

Genes (Rattus norvegicus)
Nsfl1c  (NSFL1 cofactor)
Vcp  (valosin-containing protein)

Additional Information