RGD Reference Report - The DYNLT3 light chain directly links cytoplasmic dynein to a spindle checkpoint protein, Bub3. - Rat Genome Database

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The DYNLT3 light chain directly links cytoplasmic dynein to a spindle checkpoint protein, Bub3.

Authors: Lo, KW  Kogoy, JM  Pfister, KK 
Citation: Lo KW, etal., J Biol Chem. 2007 Apr 13;282(15):11205-12. Epub 2007 Feb 7.
RGD ID: 8554144
Pubmed: PMID:17289665   (View Abstract at PubMed)
DOI: DOI:10.1074/jbc.M611279200   (Journal Full-text)

Cytoplasmic dynein is the motor protein responsible for the intracellular transport of various organelles and other cargoes toward microtubule minus ends. However, it remains to be determined how dynein is regulated to accomplish its varied roles. The dynein complex contains six subunits, including three classes of light chains. The two isoforms of the DYNLT (Tctex1) family of light chains, DYNLT1 and DYNLT3, have been proposed to link dynein to specific cargoes. However, no specific binding partner had been found for the DYNLT3 light chain. We find that DYNLT3 binds to Bub3, a spindle checkpoint protein. Bub3 binds exclusively to DYNLT3 and not to the other dynein light chains. Glutathione S-transferase pull-down and co-immunoprecipitation assays demonstrate that Bub3 interacts with the cytoplasmic dynein complex. DYNLT3 is present on kinetochores at prometaphase, but not later mitotic stages, demonstrating that this dynein light chain, like Bub3 and other checkpoint proteins, is depleted from the kinetochore during chromosome alignment. Knockdown of DYNLT3 with small interference RNA increases the mitotic index, in particular, the number of cells in prophase/prometaphase. These results demonstrate that dynein binds directly to a component of the spindle checkpoint complex through the DYNLT3 light chain. Thus, DYNLT3 contributes to dynein cargo binding specificity. These data also suggest that the subpopulation of dynein, containing the DYNLT3 light chain, may be important for chromosome congression, in addition to having a role in the transport of checkpoint proteins from the kinetochore to the spindle pole.

Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
positive regulation of mitotic cell cycle  IMP 8554144 RGD 

Cellular Component
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
cytoplasmic dynein complex  IDA 8554144 RGD 
kinetochore  IDA 8554144 RGD 
mitotic spindle astral microtubule  IDA 8554144 RGD 

Objects Annotated

Genes (Rattus norvegicus)
Dynlt3  (dynein light chain Tctex-type 3)


Additional Information