RGD Reference Report - Thy-1 functions as a signal transduction molecule in T lymphocytes and transfected B lymphocytes. - Rat Genome Database

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Thy-1 functions as a signal transduction molecule in T lymphocytes and transfected B lymphocytes.

Authors: Kroczek, RA  Gunter, KC  Germain, RN  Shevach, EM 
Citation: Kroczek RA, etal., Nature. 1986 Jul 10-16;322(6075):181-4.
RGD ID: 8554039
Pubmed: PMID:2873512   (View Abstract at PubMed)
DOI: DOI:10.1038/322181a0   (Journal Full-text)

Thy-1, a glycoprotein of relative molecular mass 25,000 (25K), is a major constituent of the cell surface of mouse thymocytes, peripheral T cells and neurones. In man, Thy-1 is present on neurones and on a small percentage of thymocytes, but is absent from peripheral T cells. The amino-acid and complementary DNA sequences of Thy-1 indicate that it has a structure similar to an isolated V (variable region) domain of immunoglobulin. Although the function of Thy-1 is unknown, the ability of different anti-Thy-1 monoclonal antibodies to activate murine T cells or induce functional changes in neuronal cells in vitro suggests that Thy-1 is involved in transmembrane signalling. We now show that crosslinking of murine Thy-1 triggers a rapid rise in the cytoplasmic free calcium concentration ([Ca2+]i), not only in murine T cells and Thy-1.2-transfected human T cells, but also in murine B-lymphoma cells transfected with the murine thy-1.2 gene. These results indicate that the generation and transduction of the signal leading to the rise in [Ca2+]i is independent of the T-cell receptor and other T-cell-specific molecules. The preservation of the [Ca2+]i-modulating function of Thy-1 in various lymphoid cells of two species further suggests that the necessary signal either originates in the Thy-1 molecule itself or is generated in concert with a highly conserved molecules(s) associated with Thy-1.

Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process

Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Thy1Ratpositive regulation of release of sequestered calcium ion into cytosol involved_inIDA PMID:2873512UniProt 

Objects Annotated

Genes (Rattus norvegicus)
Thy1  (Thy-1 cell surface antigen)

Additional Information