RGD Reference Report - Heat stress modulates hepatocyte membrane proteins during endotoxemia. - Rat Genome Database

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Heat stress modulates hepatocyte membrane proteins during endotoxemia.

Authors: Przkora, R  Haslbeck, M  Jeschke, MG  Heyde, C  Ertel, W  Herndon, DN  Bolder, U 
Citation: Przkora R, etal., Burns. 2007 Aug;33(5):628-33. Epub 2007 Mar 19.
RGD ID: 8553966
Pubmed: PMID:17374454   (View Abstract at PubMed)
DOI: DOI:10.1016/j.burns.2006.08.016   (Journal Full-text)

BACKGROUND: Hepatic failure following sepsis is one of the important features of burns. Studies have shown that in septic rats, heat stress (HS) has a protective effect on bile acid transporters in hepatocyte membranes. This study investigates the influence of HS on hepatocyte membrane proteins during endotoxemia using 2D gel electrophoresis. METHODS: Endotoxemia in rats was induced by intraperitoneal injection of Escherichia coli lipopolysaccharide (LPS) (n=24), while control rats (n=24) received saline. Twelve rats from each group were exposed to HS 2h prior to LPS or saline injection by external warming to 42 degrees C for 10 min and 12 rats in each group were exposed to ambient temperature 2h prior to LPS or saline injection. Membrane fractions were extracted 12, 24 and 72 h after LPS or saline treatment. Extracted proteins were separated using 2D gel electrophoresis. The most dominant spots were analyzed by MALDI-TOF-MS. RESULTS: Two-dimensional gel electrophoresis differentially identified expressed proteins in all treatment groups. The majority of the spots developed 24h after injection. Membrane proteins; Wnt 13, ribosomal protein L14, VLCAD, BHMT and HIT-40 were found only in HS-LPS. Protein profiles of the groups returned to normal after 72 h. CONCLUSION: We propose that HS during endotoxemia changes hepatic membrane proteins expression, which are involved in metabolism.

RGD Manual Disease Annotations    Click to see Annotation Detail View
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
Heat Stress Disorders  ISOAcadvl (Rattus norvegicus)8553966; 8553966associated with EndotoxemiaRGD 
Heat Stress Disorders  IEP 8553966associated with EndotoxemiaRGD 

Objects Annotated

Genes (Rattus norvegicus)
Acadvl  (acyl-CoA dehydrogenase, very long chain)

Genes (Mus musculus)
Acadvl  (acyl-Coenzyme A dehydrogenase, very long chain)

Genes (Homo sapiens)
ACADVL  (acyl-CoA dehydrogenase very long chain)

Objects referenced in this article
Gene Zfp709 zinc finger protein 709 Rattus norvegicus

Additional Information