RGD Reference Report - Enhancer of polycomb1, a novel homeodomain only protein-binding partner, induces skeletal muscle differentiation. - Rat Genome Database

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Enhancer of polycomb1, a novel homeodomain only protein-binding partner, induces skeletal muscle differentiation.

Authors: Kee, HJ  Kim, JR  Nam, KI  Park, HY  Shin, S  Kim, JC  Shimono, Y  Takahashi, M  Jeong, MH  Kim, N  Kim, KK  Kook, H 
Citation: Kee HJ, etal., J Biol Chem. 2007 Mar 9;282(10):7700-9. Epub 2006 Dec 27.
RGD ID: 8553913
Pubmed: PMID:17192267   (View Abstract at PubMed)
DOI: DOI:10.1074/jbc.M611198200   (Journal Full-text)

Homeodomain only protein, Hop, is an unusual small protein that modulates target gene transcription without direct binding to DNA. Here we show that Hop interacts with Enhancer of Polycomb1 (Epc1), a homolog of a Drosophila polycomb group gene product that regulates transcription, to induce the skeletal muscle differentiation. Yeast two-hybrid assay with the human adult heart cDNA library revealed that Hop can associate with Epc1. The amino-terminal domain of Epc1 as well as full Epc1 physically interacted with Hop in mammalian cells and in yeast. Epc1 is highly expressed in the embryonic heart and adult skeletal muscles. Serum deprivation induced differentiation of H9c2, a myoblast cell line, into skeletal myocytes, and Epc1 was up-regulated. Differentiation of H9c2 was induced by Epc1 overexpression, although it was severely impaired in Epc1-knockdown cells. Co-transfection of Hop potentiated Epc1-induced transactivation of myogenin and myotube formation. Hop knock-out mice elicited a decrease in myosin heavy chain and myogenin expressions in skeletal muscle and showed delay in hamstring muscle healing after injury. Differentiation was impaired in skeletal myoblasts from Hop knock-out mice. These results suggest that Epc1 plays a role in the initiation of skeletal muscle differentiation, and its interaction with Hop is required for the full activity.

Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
positive regulation of striated muscle cell differentiation acts_upstream_of_or_withinIDA 8553913PMID:17192267MGI 
positive regulation of transcription by RNA polymerase II acts_upstream_of_or_withinIDA 8553913PMID:17192267MGI 

Objects Annotated

Genes (Rattus norvegicus)
Morf4l2  (mortality factor 4 like 2)


Additional Information