RGD Reference Report - Sirtuin 2, a mammalian homolog of yeast silent information regulator-2 longevity regulator, is an oligodendroglial protein that decelerates cell differentiation through deacetylating alpha-tubulin. - Rat Genome Database

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Sirtuin 2, a mammalian homolog of yeast silent information regulator-2 longevity regulator, is an oligodendroglial protein that decelerates cell differentiation through deacetylating alpha-tubulin.

Authors: Li, W  Zhang, B  Tang, J  Cao, Q  Wu, Y  Wu, C  Guo, J  Ling, EA  Liang, F 
Citation: Li W, etal., J Neurosci. 2007 Mar 7;27(10):2606-16.
RGD ID: 8553877
Pubmed: PMID:17344398   (View Abstract at PubMed)
PMCID: PMC6672490   (View Article at PubMed Central)
DOI: DOI:10.1523/JNEUROSCI.4181-06.2007   (Journal Full-text)

Silent information regulator-2 (SIR2) proteins regulate lifespan of diverse organisms, but their distribution and roles in the CNS remain unclear. Here, we show that sirtuin 2 (SIRT2), a mammalian SIR2 homolog, is an oligodendroglial cytoplasmic protein and localized to the outer and juxtanodal loops in the myelin sheath. Among cytoplasmic proteins of OLN-93 oligodendrocytes, alpha-tubulin was the main substrate of SIRT2 deacetylase. In cultured primary oligodendrocyte precursors (OLPs), SIRT2 emergence accompanied elevated alpha-tubulin acetylation and OLP differentiation into the prematurity stage. Small interfering RNA knockdown of SIRT2 increased the alpha-tubulin acetylation, myelin basic protein expression, and cell arbor complexity of OLPs. SIRT2 overexpression had the opposite effects, and counteracted the cell arborization-promoting effect of overexpressed juxtanodin. SIRT2 mutation concomitantly reduced its deacetylase activity and its impeding effect on OLP arborization. These results demonstrated a counterbalancing role of SIRT2 against a facilitatory effect of tubulin acetylation on oligodendroglial differentiation. Selective SIRT2 availability to oligodendroglia may have important implications for myelinogenesis, myelin-axon interaction, and brain aging.



Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Sirt2Ratnegative regulation of oligodendrocyte differentiation  IMP  RGD 
Sirt2Ratnegative regulation of oligodendrocyte progenitor proliferation involved_inIMP PMID:17344398UniProt 
Sirt2Rattubulin deacetylation involved_inIDA PMID:17344398UniProt 

Cellular Component

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Sirt2Ratglial cell projection located_inIDA PMID:17344398UniProt 
Sirt2Ratheterochromatin located_inIDA PMID:17344398UniProt 
Sirt2Ratjuxtaparanode region of axon located_inIDA PMID:17344398UniProt 
Sirt2Ratmyelin sheath located_inIDA PMID:17344398UniProt 
Sirt2Ratmyelin sheath abaxonal region  IDA  RGD 
Sirt2Ratparanodal junction  IDA  RGD 
Sirt2Ratparanode region of axon located_inIDA PMID:17344398UniProt 
Sirt2Ratperikaryon located_inIDA PMID:17344398UniProt 
Sirt2Ratterminal loop  IDA  RGD 

Molecular Function

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Sirt2RatNAD-dependent protein lysine deacetylase activity enablesIDA PMID:17344398UniProt 
Sirt2Rattubulin deacetylase activity enablesIDA PMID:17344398UniProt 

Objects Annotated

Genes (Rattus norvegicus)
Sirt2  (sirtuin 2)


Additional Information