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Rev-erbalpha regulates circadian rhythms and StAR expression in rat granulosa cells as identified by the agonist GSK4112.

Authors: Chen, H  Chu, G  Zhao, L  Yamauchi, N  Shigeyoshi, Y  Hashimoto, S  Hattori, MA 
Citation: Chen H, etal., Biochem Biophys Res Commun. 2012 Apr 6;420(2):374-9. doi: 10.1016/j.bbrc.2012.02.164. Epub 2012 Mar 8.
Pubmed: (View Article at PubMed) PMID:22425774
DOI: Full-text: DOI:10.1016/j.bbrc.2012.02.164

The Rev-erbalpha gene is regarded as a circadian clock gene and clock-regulated gene which regulates the circadian transcriptional/translational loop in a subtle way. Here, we first detected the circadian oscillation in mature granulosa cells from antral follicles using a real-time monitoring system of Per2 promoter activity with the addition of FSH. Then we used GSK4112, an agonist ligand of Rev-erbalpha, to investigate the function of Rev-erbalpha. GSK4112 treatment significantly reduced the Per2-dLuc amplitude and induced the Per2 oscillation phase advance shift. GSK4112 significantly inhibited Bmal1 mRNA expression, whereas it did clearly stimulate expression of StAR mRNA in a dose-dependent manner. Our data are the first to show the Rev-erbalpha function in the steroid biosynthesis of rat granulosa cells, and to suggest that Rev-erbalpha may coordinate circadian rhythm and metabolism in rat ovaries.


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RGD Object Information
RGD ID: 8553819
Created: 2014-05-08
Species: All species
Last Modified: 2014-05-08
Status: ACTIVE


RGD is funded by grant HL64541 from the National Heart, Lung, and Blood Institute on behalf of the NIH.