RGD Reference Report - Myopalladin, a novel 145-kilodalton sarcomeric protein with multiple roles in Z-disc and I-band protein assemblies. - Rat Genome Database

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Myopalladin, a novel 145-kilodalton sarcomeric protein with multiple roles in Z-disc and I-band protein assemblies.

Authors: Bang, ML  Mudry, RE  McElhinny, AS  Trombitas, K  Geach, AJ  Yamasaki, R  Sorimachi, H  Granzier, H  Gregorio, CC  Labeit, S 
Citation: Bang ML, etal., J Cell Biol. 2001 Apr 16;153(2):413-27.
RGD ID: 8553772
Pubmed: PMID:11309420   (View Abstract at PubMed)
PMCID: PMC2169455   (View Article at PubMed Central)

We describe here a novel sarcomeric 145-kD protein, myopalladin, which tethers together the COOH-terminal Src homology 3 domains of nebulin and nebulette with the EF hand motifs of alpha-actinin in vertebrate Z-lines. Myopalladin's nebulin/nebulette and alpha-actinin-binding sites are contained in two distinct regions within its COOH-terminal 90-kD domain. Both sites are highly homologous with those found in palladin, a protein described recently required for actin cytoskeletal assembly (Parast, M.M., and C.A. Otey. 2000. J. Cell Biol. 150:643-656). This suggests that palladin and myopalladin may have conserved roles in stress fiber and Z-line assembly. The NH(2)-terminal region of myopalladin specifically binds to the cardiac ankyrin repeat protein (CARP), a nuclear protein involved in control of muscle gene expression. Immunofluorescence and immunoelectron microscopy studies revealed that myopalladin also colocalized with CARP in the central I-band of striated muscle sarcomeres. Overexpression of myopalladin's NH(2)-terminal CARP-binding region in live cardiac myocytes resulted in severe disruption of all sarcomeric components studied, suggesting that the myopalladin-CARP complex in the central I-band may have an important regulatory role in maintaining sarcomeric integrity. Our data also suggest that myopalladin may link regulatory mechanisms involved in Z-line structure (via alpha-actinin and nebulin/nebulette) to those involved in muscle gene expression (via CARP).

Gene Ontology Annotations    Click to see Annotation Detail View

Cellular Component
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
I band located_inIDA 8553772; 8553772PMID:11309420BHF-UCL 
nucleus located_inIDA 8553772; 8553772PMID:11309420BHF-UCL 
Z disc located_inIDA 8553772PMID:11309420BHF-UCL 

Objects Annotated

Genes (Rattus norvegicus)
Ankrd1  (ankyrin repeat domain 1)
Mypn  (myopalladin)


Additional Information