RGD Reference Report - The c-Myc target gene Rcl (C6orf108) encodes a novel enzyme, deoxynucleoside 5'-monophosphate N-glycosidase. - Rat Genome Database

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The c-Myc target gene Rcl (C6orf108) encodes a novel enzyme, deoxynucleoside 5'-monophosphate N-glycosidase.

Authors: Ghiorghi, YK  Zeller, KI  Dang, CV  Kaminski, PA 
Citation: Ghiorghi YK, etal., J Biol Chem. 2007 Mar 16;282(11):8150-6. Epub 2007 Jan 18.
RGD ID: 8553712
Pubmed: PMID:17234634   (View Abstract at PubMed)
DOI: DOI:10.1074/jbc.M610648200   (Journal Full-text)

RCL is a c-Myc target with tumorigenic potential. Genome annotation predicted that RCL belonged to the N-deoxyribosyltransferase family. However, its putative relationship to this class of enzymes did not lead to its precise biochemical function. The purified native or N-terminal His-tagged recombinant rat RCL protein expressed in Escherichia coli exhibits the same enzyme activity, deoxynucleoside 5'-monophosphate N-glycosidase, never before described. dGMP appears to be the best substrate. RCL opens a new route in the nucleotide catabolic pathways by cleaving the N-glycosidic bond of deoxynucleoside 5'-monophosphates to yield two reaction products, deoxyribose 5-phosphate and purine or pyrimidine base. Biochemical studies show marked differences in the terms of the structure and catalytic mechanism between RCL and of its closest enzyme family neighbor, N-deoxyribosyltransferase. The reaction products of this novel enzyme activity have been implicated in purine or pyrimidine salvage, glycolysis, and angiogenesis, and hence are all highly relevant for tumorigenesis.



Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Dnph1Ratallantoin metabolic process involved_inIDA PMID:17234634MGI 
Dnph1Ratdeoxyribonucleoside monophosphate catabolic process involved_inIDA PMID:17234634UniProt 
Dnph1RatdGMP catabolic process involved_inIDA PMID:17234634MGI 
Dnph1Ratpositive regulation of cell growth involved_inIDA PMID:17234634UniProt 

Molecular Function

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Dnph1Rat5-hydroxymethyl-dUMP N-hydrolase activity enablesIDA PMID:17234634MGI 
Dnph1Ratprotein homodimerization activity enablesIDA PMID:17234634UniProt 

Objects Annotated

Genes (Rattus norvegicus)
Dnph1  (2'-deoxynucleoside 5'-phosphate N-hydrolase 1)


Additional Information