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All-trans retinoic acid is a ligand for the orphan nuclear receptor ROR beta.

Authors: Stehlin-Gaon, C  Willmann, D  Zeyer, D  Sanglier, S  Van Dorsselaer, A  Renaud, JP  Moras, D  Schule, R 
Citation: Stehlin-Gaon C, etal., Nat Struct Biol. 2003 Oct;10(10):820-5. Epub 2003 Sep 7.
Pubmed: (View Article at PubMed) PMID:12958591
DOI: Full-text: DOI:10.1038/nsb979

Retinoids regulate gene expression through binding to the nuclear retinoic acid receptors (RARs) and retinoid X receptors (RXRs). In contrast, no ligands for the retinoic acid receptor-related orphan receptors beta and gamma (ROR beta and gamma) have been identified, yet structural data and structure-function analyses indicate that ROR beta is a ligand-regulated nuclear receptor. Using nondenaturing mass spectrometry and scintillation proximity assays we found that all-trans retinoic acid (ATRA) and several retinoids bind to the ROR beta ligand-binding domain (LBD). The crystal structures of the complex with ATRA and with the synthetic analog ALRT 1550 reveal the binding modes of these ligands. ATRA and related retinoids inhibit ROR beta but not ROR alpha transcriptional activity suggesting that high-affinity, subtype-specific ligands could be designed for the identification of ROR beta target genes. Our results identify ROR beta as a retinoid-regulated nuclear receptor, providing a novel pathway for retinoid action.

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RGD Object Information
RGD ID: 8553609
Created: 2014-05-08
Species: All species
Last Modified: 2014-05-08
Status: ACTIVE



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RGD is funded by grant HL64541 from the National Heart, Lung, and Blood Institute on behalf of the NIH.