RGD Reference Report - Glypican-1 and alpha4(V) collagen are required for Schwann cell myelination. - Rat Genome Database

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Glypican-1 and alpha4(V) collagen are required for Schwann cell myelination.

Authors: Chernousov, MA  Rothblum, K  Stahl, RC  Evans, A  Prentiss, L  Carey, DJ 
Citation: Chernousov MA, etal., J Neurosci. 2006 Jan 11;26(2):508-17.
RGD ID: 8553537
Pubmed: PMID:16407548   (View Abstract at PubMed)
PMCID: PMC6674409   (View Article at PubMed Central)
DOI: DOI:10.1523/JNEUROSCI.2544-05.2006   (Journal Full-text)

Schwann cell myelination requires interactions with the extracellular matrix (ECM) mediated by cell surface receptors. Previously, we identified a type V collagen family member, alpha4(V) collagen, which is expressed by Schwann cells during peripheral nerve differentiation. This collagen binds with high affinity to heparan sulfate through a unique binding motif in the noncollagenous N-terminal domain (NTD). The principal alpha4(V) collagen-binding protein on the Schwann cell surface is the heparan sulfate proteoglycan glypican-1. We investigated the role of alpha4(V) collagen and glypican-1 in Schwann cell terminal differentiation in cultures of Schwann cells and dorsal root ganglion neurons. Small interfering RNA-mediated suppression of glypican-1 expression decreased binding of alpha4(V)-NTD to Schwann cells, adhesion and spreading of Schwann cells on alpha4(V)-NTD, and incorporation of alpha4(V) collagen into Schwann cell ECM. In cocultures, alpha4(V) collagen coassembles with laminin on the surface of polarized Schwann cells to form tube-like ECM structures that are sites of myelination. Suppression of glypican-1 or alpha4(V) collagen expression significantly inhibited myelination. These results demonstrate an important role for these proteins in peripheral nerve terminal differentiation.

Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
myelin assembly involved_inIDA 8553537PMID:16407548UniProt 
Schwann cell differentiation involved_inIDA 8553537PMID:16407548UniProt 

Objects Annotated

Genes (Rattus norvegicus)
Gpc1  (glypican 1)


Additional Information