RGD Reference Report - Members of the E2D (UbcH5) family mediate the ubiquitination of the conserved cysteine of Pex5p, the peroxisomal import receptor. - Rat Genome Database

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Members of the E2D (UbcH5) family mediate the ubiquitination of the conserved cysteine of Pex5p, the peroxisomal import receptor.

Authors: Grou, CP  Carvalho, AF  Pinto, MP  Wiese, S  Piechura, H  Meyer, HE  Warscheid, B  Sa-Miranda, C  Azevedo, JE 
Citation: Grou CP, etal., J Biol Chem. 2008 May 23;283(21):14190-7. doi: 10.1074/jbc.M800402200. Epub 2008 Mar 22.
RGD ID: 8553433
Pubmed: PMID:18359941   (View Abstract at PubMed)
DOI: DOI:10.1074/jbc.M800402200   (Journal Full-text)

According to current models of peroxisomal biogenesis, newly synthesized peroxisomal matrix proteins are transported into the organelle by Pex5p. Pex5p recognizes these proteins in the cytosol, mediates their membrane translocation, and is exported back into the cytosol in an ATP-dependent manner. We have previously shown that export of Pex5p is preceded by (and requires) monoubiquitination of a conserved cysteine residue present at its N terminus. In yeasts, and probably also in plants, ubiquitination of Pex5p is mediated by a specialized ubiquitin-conjugating enzyme, Pex4p. In mammals, the identity of this enzyme has remained unknown for many years. Here, we provide evidence suggesting that E2D1/2/3 (UbcH5a/b/c) are the mammalian functional counterparts of yeast/plant Pex4p. The mechanistic implications of these findings are discussed.

Gene Ontology Annotations    Click to see Annotation Detail View

Molecular Function
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
ubiquitin-protein transferase activity enablesIDA 8553433; 8553433PMID:18359941UniProt 

Objects Annotated

Genes (Rattus norvegicus)
Ube2d1  (ubiquitin-conjugating enzyme E2D 1)
Ube2d2  (ubiquitin-conjugating enzyme E2D 2)


Additional Information