RGD Reference Report - Involvement of protein kinase C beta 2 in c-myc induction by high glucose in pancreatic beta-cells. - Rat Genome Database

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Involvement of protein kinase C beta 2 in c-myc induction by high glucose in pancreatic beta-cells.

Authors: Kaneto, H  Suzuma, K  Sharma, A  Bonner-Weir, S  King, GL  Weir, GC 
Citation: Kaneto H, etal., J Biol Chem. 2002 Feb 1;277(5):3680-5. Epub 2001 Nov 19.
RGD ID: 8553365
Pubmed: PMID:11714718   (View Abstract at PubMed)
DOI: DOI:10.1074/jbc.M109647200   (Journal Full-text)

The expression of the basic helix-loop-helix transcription factor c-Myc is induced in pancreatic islets of several different diabetic model animals and is possibly involved in suppression of the insulin gene transcription. In this study, we found that activity of protein kinase C is increased by high glucose, preceding the induction of c-myc expression and that PKC beta2 specifically regulates c-myc expression in pancreatic beta-cells. Since PKC alpha, beta2, delta, epsilon, and zeta were expressed in rat pancreatic islets, we prepared each wild type (WT) and dominant negative type (DN) PKC isoform (alpha, beta2, delta, epsilon, and zeta)-expressing adenovirus to examine the effect of each PKC isoform on c-myc expression. In isolated rat pancreatic islets, adenovirus-mediated overexpression of WT PKC beta2, but not other PKC isoforms, markedly increased c-myc expression. Moreover, c-myc induction by high glucose was suppressed by adenovirus-mediated overexpression of DN PKC beta2 but not by other DN PKC isoforms. Finally, adenovirus-mediated overexpression of WT PKC beta2, but not of other PKC isoforms, leads to suppression of the insulin gene transcription in pancreatic islets. These results suggest that at least some of the reduction of insulin gene transcription found in the diabetic state is mediated by PKC beta2 regulation of c-myc expression.

Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
negative regulation of insulin receptor signaling pathway involved_inIDA 8553365PMID:11714718UniProt 

Objects Annotated

Genes (Rattus norvegicus)
Prkcb  (protein kinase C, beta)


Additional Information