RGD Reference Report - Expression of Toll-Like Receptors 2, 4 and Nuclear Factor Kappa B in Mucosal Lesions of Human Otitis: Pattern and Relationship in a Clinical Immunohistochemical Study. - Rat Genome Database

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Expression of Toll-Like Receptors 2, 4 and Nuclear Factor Kappa B in Mucosal Lesions of Human Otitis: Pattern and Relationship in a Clinical Immunohistochemical Study.

Authors: Jesic, S  Jotic, A  Tomanovic, N  Zivkovic, M  Kolakovic, A  Stankovic, A 
Citation: Jesic S, etal., Ann Otol Rhinol Laryngol. 2014 Apr 1.
RGD ID: 8552995
Pubmed: (View Article at PubMed) PMID:24690988
DOI: Full-text: DOI:10.1177/0003489414527229

OBJECTIVES: The objectives were to detect and compare the expression of toll-like receptors (TLRs) 2, 4 and nuclear factor kappa B in mucosal lesions of chronic otitis. METHODS: Fifty-five tissue samples obtained from children and adults operated on for otitis were investigated by semiquantitative immunohistochemical methods using polyclonal antibodies for TLR 2, 4 and NFkB. Kruskal-Wallis, Mann-Whitney, and Kendall's tau rank correlation tests were used. RESULTS: Stronger expression of TLR2, 4 was found in inflamed mucosa than in the control for children and adults (TLR2: H = 23.86, P < .001; TLR4: H = 22.80, P < .001) (TLR2: H = 17.53, P < .001; TLR4: H = 11.99, P < .001); in cholesteatoma perimatrix compared to tubotympanic lesions in children (TLR2: H = 11.06, P = .004; TLR4: H = 10.61, P = .005) and adults (TLR2: H = 10.73, P = .013; TLR4: H = 9.65, P = .021). No differences were found in NFkB expression (H = 0.042, P = .99). Significant correlations were found for all pairs of molecules in cholesteatoma and tubotympanic mucosa of adults (TLR2, 4: P = .002, P < .001; TLR2-NfkB: P = .032, P = .021; TLR4-NFkB: P = .035, P = .0013), only TLR4-NFkB in tubotympanic otitis of children (P = .026). CONCLUSIONS: Toll-like receptors 2, 4 and NFkB mediate inflammation in cholesteatoma and mucosal lesions of tubotympanic otitis in children and adults. Significant correlations between all pairs of molecules in all samples were detected in adults, but only TLR4-NFkB in children.

Annotation

Disease Annotations    

Objects Annotated

Genes (Rattus norvegicus)
Rela  (RELA proto-oncogene, NF-kB subunit)
Tlr2  (toll-like receptor 2)
Tlr4  (toll-like receptor 4)

Genes (Mus musculus)
Rela  (v-rel reticuloendotheliosis viral oncogene homolog A (avian))
Tlr2  (toll-like receptor 2)
Tlr4  (toll-like receptor 4)

Genes (Homo sapiens)
RELA  (RELA proto-oncogene, NF-kB subunit)
TLR2  (toll like receptor 2)
TLR4  (toll like receptor 4)


Additional Information