RGD Reference Report - A single amino acid substitution (Cys249Trp) in Crb1 causes retinal degeneration and deregulates expression of pituitary tumor transforming gene Pttg1. - Rat Genome Database

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A single amino acid substitution (Cys249Trp) in Crb1 causes retinal degeneration and deregulates expression of pituitary tumor transforming gene Pttg1.

Authors: Van de Pavert, SA  Meuleman, J  Malysheva, A  Aartsen, WM  Versteeg, I  Tonagel, F  Kamphuis, W  McCabe, CJ  Seeliger, MW  Wijnholds, J 
Citation: van de Pavert SA, etal., J Neurosci. 2007 Jan 17;27(3):564-73.
RGD ID: 8552692
Pubmed: PMID:17234588   (View Abstract at PubMed)
PMCID: PMC6672796   (View Article at PubMed Central)
DOI: DOI:10.1523/JNEUROSCI.3496-06.2007   (Journal Full-text)

Different mutations in the human Crumbs homolog-1 (CRB1) gene cause a variety of retinal dystrophies, such as Leber congenital amaurosis, early onset retinitis pigmentosa (e.g., RP12), RP with Coats-like exudative vasculopathy, and pigmented paravenous retinochoroidal atrophy. Loss of Crb1 leads to displaced photoreceptors and focal degeneration of all neural layers attributable to loss of adhesion between photoreceptors and Muller glia cells. To gain insight into genotype-phenotype relationship, we generated Crb1(C249W) mice that harbor an amino acid substitution (Cys249Trp) in the extracellular sixth calcium-binding epidermal growth factor domain of Crb1. Our analysis showed that Crb1(C249W) as wild-type protein trafficked to the subapical region adjacent to adherens junctions at the outer limiting membrane (OLM). Hence, these data suggest correct trafficking of the corresponding mutant CRB1 in RP12 patients. Crb1(C249W) mice showed loss of photoreceptors in the retina, relatively late compared with mice lacking Crb1. Scanning laser ophthalmoscopy revealed autofluorescent dots that presumably represent layer abnormalities after OLM disturbance. Gene expression analyses revealed lower levels of pituitary tumor transforming gene 1 (Pttg1) transcripts in Crb1(C249W/-) knock-in and Crb1(-/-) knock-out compared with control retinas. Exposure to white light decreased levels of Pttg1 in Crb1 mutant retinas. We hypothesize deregulation of Pttg1 expression attributable to a C249W substitution in the extracellular domain of Crb1.



RGD Manual Disease Annotations    Click to see Annotation Detail View

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
CRB1Humanlate-onset retinal degeneration  ISOCrb1 (Mus musculus)DNA:missense mutation: :p.C249W (mouse)RGD 
Crb1Ratlate-onset retinal degeneration  ISOCrb1 (Mus musculus)DNA:missense mutation: :p.C249W (mouse)RGD 
Crb1Mouselate-onset retinal degeneration  IAGP DNA:missense mutation: :p.C249W (mouse)RGD 

Objects Annotated

Genes (Rattus norvegicus)
Crb1  (crumbs cell polarity complex component 1)

Genes (Mus musculus)
Crb1  (crumbs family member 1, photoreceptor morphogenesis associated)

Genes (Homo sapiens)
CRB1  (crumbs cell polarity complex component 1)


Additional Information