RGD Reference Report - Prostaglandin E(2) enhances proliferation, dedifferentiation and stem-like properties of rat retinal Muller glial cells in vitro. - Rat Genome Database

Send us a Message
Submit Data |  Help |  Video Tutorials |  News |  Publications |  Download |  REST API |  Citing RGD |  Contact   
Search RGD Grant Resources Citing RGD About Us Contact Us
Genes Variants Community Projects QTLs Strains Markers Genome Information Ontologies Cell Lines References Download Submit Data
OntoMate (Literature Search) JBrowse (Genome Browser) Synteny Browser (VCMap) Variant Visualizer Multi-Ontology Enrichment (MOET) Gene-Ortholog Location Finder (GOLF) InterViewer (Protein-Protein Interactions) PhenoMiner (Quatitative Phenotypes) Gene Annotator OLGA (Gene List Generator) AllianceMine GViewer (Genome Viewer)
Aging & Age-Related Disease Cancer & Neoplastic Disease Cardiovascular Disease Coronavirus Disease Developmental Disease Diabetes Hematologic Disease Immune & Inflammatory Disease Infectious Disease Liver Disease Neurological Disease Obesity & Metabolic Syndrome Renal Disease Respiratory Disease Sensory Organ Disease
Find Models Genetic Models Autism Models Rat PhenoMiner (Quantitative Phenotypes) Chinchilla PhenoMiner Expected Ranges (Quantitative Phenotype) PhenoMiner Term Comparison Hybrid Rat Diversity Panel Phenotypes Phenotypes in Other Animal Models Animal Husbandry Strain Medical Records Phylogenetics Strain Availability Calendar Rats 101 Submissions Photo Archive
Pathways
Rat Community Forum Directory of Rat Laboratories Video Tutorials News RGD Publications RGD Presentations Archive Nomenclature Guidelines Resource Links Laboratory Resources Employment Resources
Advanced Search (OLGA)

Prostaglandin E(2) enhances proliferation, dedifferentiation and stem-like properties of rat retinal Muller glial cells in vitro.

Authors: Wang, L  Lang, LL  Wang, Y  Shi, S  Liu, L 
Citation: Wang L, etal., Ophthalmic Res. 2013;49(2):100-7. doi: 10.1159/000345256. Epub 2012 Dec 18.
RGD ID: 8552382
Pubmed: PMID:23257891   (View Abstract at PubMed)
DOI: DOI:10.1159/000345256   (Journal Full-text)

AIMS: To investigate the differences of gene expression in rat retinal Muller glial cells after basic fibroblast growth factor (bFGF) treatment, and to explore the function of prostaglandin E(2) (PGE(2)) in the proliferation and dedifferentiation of rat retinal Muller glial cells. MATERIALS AND METHODS: A gene expression profile in rat retinal Muller glial cells after bFGF treatment, matching untreated cells, was conducted using gene array technology. The candidate gene selection was performed on GenMAPP/MAPPFinder. The functional effects of PGE(2) on the proliferation and dedifferentiation of Muller cells were further investigated. RESULTS: Gene array analysis identified that 298 genes were upregulated and 293 genes were downregulated. The GenMAPP/MAPPFinder results showed that the PG biosynthetic process had the highest correlation (Z score 8.803) with the stem cell characteristics of Muller cells. The quantitative real-time reverse transcription polymerase chain reaction (qRT-PCR) results showed that PGE(2) can significantly upregulate the expression of cyclin D(1) in Muller cells. Also, the bromodeoxyuridine and flow cytometry results showed that PGE(2) can significantly increase the proportion of Muller cells in S phase. Furthermore, qRT-PCR showed that the expressions of nestin (a marker of neural stem/precursor cells) and Pax6 (a marker of retinal stem/precursor cells) were significantly upregulated by PGE(2) stimulation, and similar results were obtained by immunofluorescence staining. CONCLUSION: PGE(2) may enhance the proliferation, dedifferentiation, and stem-like properties of rat retinal Muller glial cells in vitro.



Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Pax6Ratcellular response to prostaglandin E stimulus  IEP  RGD 

Objects Annotated

Genes (Rattus norvegicus)
Pax6  (paired box 6)


Additional Information