RGD Reference Report - Silencing rapsyn in vivo decreases acetylcholine receptors and augments sodium channels and secondary postsynaptic membrane folding. - Rat Genome Database

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Silencing rapsyn in vivo decreases acetylcholine receptors and augments sodium channels and secondary postsynaptic membrane folding.

Authors: Martinez-Martinez, P  Phernambucq, M  Steinbusch, L  Schaeffer, L  Berrih-Aknin, S  Duimel, H  Frederik, P  Molenaar, P  De Baets, MH  Losen, M 
Citation: Martinez-Martinez P, etal., Neurobiol Dis. 2009 Jul;35(1):14-23. doi: 10.1016/j.nbd.2009.03.008. Epub 2009 Apr 1.
RGD ID: 8549750
Pubmed: PMID:19344765   (View Abstract at PubMed)
DOI: DOI:10.1016/j.nbd.2009.03.008   (Journal Full-text)

The receptor-associated protein of the synapse (rapsyn) is required for anchoring and stabilizing the nicotinic acetylcholine receptor (AChR) in the postsynaptic membrane of the neuromuscular junction (NMJ) during development. Here we studied the role of rapsyn in the maintenance of the adult NMJ by reducing rapsyn expression levels with short hairpin RNA (shRNA). Silencing rapsyn led to the average reduction of the protein levels of rapsyn (31% loss) and AChR (36% loss) at the NMJ within 2 weeks, corresponding to previously reported half life of these proteins. On the other hand, the sodium channel protein expression was augmented (66%) in rapsyn-silenced muscles. Unexpectedly, at the ultrastructural level a significant increase in the amount of secondary folds of the postsynaptic membrane in silenced muscles was observed. The neuromuscular transmission in rapsyn-silenced muscles was mildly impaired. The results suggest that the adult NMJ can rapidly produce postsynaptic folds to compensate for AChR and rapsyn loss.

RGD Manual Disease Annotations    Click to see Annotation Detail View
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
Experimental Autoimmune Myasthenia Gravis  ISORapsn (Rattus norvegicus)8549750; 8549750protein:decreased expression:neuromuscular junctionRGD 
Experimental Autoimmune Myasthenia Gravis  IEP 8549750protein:decreased expression:neuromuscular junctionRGD 

Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process

Cellular Component
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
neuromuscular junction  IDA 8549750 RGD 

Objects Annotated

Genes (Rattus norvegicus)
Rapsn  (receptor-associated protein of the synapse)

Genes (Mus musculus)
Rapsn  (receptor-associated protein of the synapse)

Genes (Homo sapiens)
RAPSN  (receptor associated protein of the synapse)


Additional Information