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Spinal injection of TNF-alpha-activated astrocytes produces persistent pain symptom mechanical allodynia by releasing monocyte chemoattractant protein-1.

Authors: Gao, YJ  Zhang, L  Ji, RR 
Citation: Gao YJ, etal., Glia. 2010 Nov 15;58(15):1871-80. doi: 10.1002/glia.21056.
Pubmed: (View Article at PubMed) PMID:20737477
DOI: Full-text: DOI:10.1002/glia.21056

Accumulating evidence suggests that spinal astrocytes play an important role in the genesis of persistent pain, by increasing the activity of spinal cord nociceptive neurons, i.e., central sensitization. However, direct evidence of whether activation of astrocytes is sufficient to induce chronic pain symptoms is lacking. We investigated whether and how spinal injection of activated astrocytes could produce mechanical allodynia, a cardinal feature of chronic pain, in naive mice. Spinal (intrathecal) injection of astrocytes, which were prepared from cerebral cortexes of neonatal mice and briefly stimulated by tumor necrosis factor-alpha (TNF-alpha), induced a substantial decrease in paw withdrawal thresholds, indicating the development of mechanical allodynia. This allodynia was prevented when the astrocyte cultures were pretreated with a peptide inhibitor of c-Jun N-terminal kinase (JNK), D-JNKI-1. Of note a short exposure of astrocytes to TNF-alpha for 15 min dramatically increased the expression and release of the chemokine monocyte chemoattractant protein-1 (MCP-1), even 3 h after TNF-alpha withdrawal, in a JNK-dependent manner. In parallel, intrathecal administration of TNF-alpha induced MCP-1 expression in spinal cord astrocytes. In particular, mechanical allodynia induced by TNF-alpha-activated astrocytes was reversed by a MCP-1 neutralizing antibody. Finally, pretreatment of astrocytes with MCP-1 siRNA attenuated astrocytes-induced mechanical allodynia. Taken together, our results suggest that activated astrocytes are sufficient to produce persistent pain symptom in naive mice by releasing MCP-1.

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RGD ID: 8548848
Created: 2014-03-24
Species: All species
Last Modified: 2014-03-24
Status: ACTIVE



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RGD is funded by grant HL64541 from the National Heart, Lung, and Blood Institute on behalf of the NIH.