RGD Reference Report - Graves' orbitopathy results in profound changes in tear composition: a study of plasminogen activator inhibitor-1 and seven cytokines. - Rat Genome Database

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Graves' orbitopathy results in profound changes in tear composition: a study of plasminogen activator inhibitor-1 and seven cytokines.

Authors: Ujhelyi, B  Gogolak, P  Erdei, A  Nagy, V  Balazs, E  Rajnavolgyi, E  Berta, A  Nagy, EV 
Citation: Ujhelyi B, etal., Thyroid. 2012 Apr;22(4):407-14. doi: 10.1089/thy.2011.0248. Epub 2012 Mar 2.
RGD ID: 8547756
Pubmed: PMID:22385289   (View Abstract at PubMed)
DOI: DOI:10.1089/thy.2011.0248   (Journal Full-text)

BACKGROUND: Secretion of cytokines and expression of cytokine receptors have been reported in the orbital connective tissue in Graves' orbitopathy (GO). Lacrimal glands are putative autoimmune targets, and changes in tear film and ocular surface have also been described. Our aim was to characterize the cytokine profile of tears in patients with Graves' disease (GD) with and without orbitopathy. METHODS: Tear samples were collected from 54 eyes of GO patients (age 43.4+/-15.2 years), 18 eyes of GD patients (age 46.8+/-11.7 years), and 24 control eyes (age 38.6+/-13.8 years). Patients underwent ophthalmological examination including Clinical Activity Score (CAS). The level of interleukin (IL)-1beta, IL-6, IL-13, IL-17A, IL-18, tumor necrosis factor (TNF)-alpha, and RANTES (regulated upon activation, normal T-cell expressed, and secreted) as well as plasminogen activator inhibitor-1 (PAI-1) were measured by multiplex bead array and release values were calculated. RESULTS: The release of IL-1beta, IL-6, IL-13, IL-17A, IL-18, TNF-alpha, and RANTES were significantly higher in GO patients compared to controls (p<0.05). There was a 2.5-fold increase of IL-6 release. No significant differences were found in cytokine release between the GO and GD groups. In the GO group, significant positive correlation was found between CAS and the release of IL-6 and PAI-1 into tears (r=0.27, p<0.05 and r=0.24, p<0.05, respectively). PAI-1 release was significantly higher in GO than in GD patients and was increased in both the GD and GO groups compared to controls. CONCLUSIONS: Impaired cytokine balance has been observed in tears of GO patients. Secretion of IL-6 into tears might be a useful indicator of disease activity in GO.

RGD Manual Disease Annotations    Click to see Annotation Detail View
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
Graves ophthalmopathy severityIEP 8547756associated with Graves Disease and protein:increased expression:tears (human)RGD 
Graves ophthalmopathy severityISOSERPINE1 (Homo sapiens)8547756; 8547756associated with Graves Disease and protein:increased expression:tears (human)RGD 
Graves' disease  IEP 8547756protein:increased expression:tears (human)RGD 
Graves' disease  ISOSERPINE1 (Homo sapiens)8547756; 8547756protein:increased expression:tears (human)RGD 

Objects Annotated

Genes (Rattus norvegicus)
Serpine1  (serpin family E member 1)

Genes (Mus musculus)
Serpine1  (serine (or cysteine) peptidase inhibitor, clade E, member 1)

Genes (Homo sapiens)
SERPINE1  (serpin family E member 1)


Additional Information