RGD Reference Report - Dynamic changes of mitochondrial fusion and fission proteins after transient cerebral ischemia in mice. - Rat Genome Database

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Dynamic changes of mitochondrial fusion and fission proteins after transient cerebral ischemia in mice.

Authors: Liu, W  Tian, F  Kurata, T  Morimoto, N  Abe, K 
Citation: Liu W, etal., J Neurosci Res. 2012 Jun;90(6):1183-9. doi: 10.1002/jnr.23016. Epub 2012 Feb 16.
RGD ID: 7800722
Pubmed: PMID:22345048   (View Abstract at PubMed)
DOI: DOI:10.1002/jnr.23016   (Journal Full-text)

With fusion or fission, mitochondria alter their morphology in response to various physiological and pathological stimuli, resulting in elongated, tubular, interconnected, or fragmented forms. Immunohistochemistry and Western blot analysis were performed at 2 days, 7 days, 14 days, and 28 days after 90 min of transient middle cerebral artery occlusion (tMCAO) in mice. This study showed that mitochondrial fission protein dynamin-related protein 1 (Drp1) and fusion protein optic atrophy 1 (Opa1) were both upregulated in the ischemic penumbra, with the peak at 2 days after tMCAO, whereas phosphorylated-Drp1 (P-Drp1) progressively increased with a peak at 14 days after tMCAO. Double-immunofluorescence analysis showed many Drp1/cytochrome c oxidase subunit l (COX1) double-positive cells and Opa1/COX1 double-positive cells in the ischemic penumbra and also showed some double-positive cells with Drp1/terminal deoxynucleotidyl transferase-mediated dUTP-digoxigenin nick end labeling (TUNEL) and Opa1/TUNEL in the ischemic penumbra. In contrast, both Drp1 and Opa1 showed progressive decreases until 2 days after tMCAO in the ischemic core because of necrotic brain damage. The present study suggests that there was a continuous mitochondrial fission and fusion during these periods in the ischemic penumbra after tMCAO, probably in an effort toward mitophagy and cellular survival.

RGD Manual Disease Annotations    Click to see Annotation Detail View
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
transient cerebral ischemia  ISOOpa1 (Mus musculus)7800722; 7800722protein:altered expression:brainRGD 
transient cerebral ischemia  IEP 7800722protein:altered expression:brainRGD 

Objects Annotated

Genes (Rattus norvegicus)
Opa1  (OPA1, mitochondrial dynamin like GTPase)

Genes (Mus musculus)
Opa1  (OPA1, mitochondrial dynamin like GTPase)

Genes (Homo sapiens)
OPA1  (OPA1 mitochondrial dynamin like GTPase)


Additional Information