RGD Reference Report - [Differential expression of toll-like receptors in chronic suppurative otitis media and cholesteatoma]. - Rat Genome Database

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[Differential expression of toll-like receptors in chronic suppurative otitis media and cholesteatoma].

Authors: Si, Y  Zhang, ZG  Huang, CY  He, JF  Feng, LQ  Chen, YB  Chen, T  Huang, X 
Citation: Si Y, etal., Zhonghua Er Bi Yan Hou Tou Jing Wai Ke Za Zhi. 2012 May;47(5):388-93.
RGD ID: 7800662
Pubmed: PMID:22883581   (View Abstract at PubMed)

OBJECTIVE: To investigate the differential expression of toll-like receptor 2 (TLR2), toll-like receptor 4 (TLR4) and their potential role in the pathogenesis of chronic suppurative otitis meida and cholesteatoma. METHODS: Normal cannal skin of 30 patients with tympanosclerosis were enrolled as control, 30 cases with choronic suppurative otitis media and 30 patients with cholesteatoma were studied. Real-time PCR, Western blot and Immunohistochemistry were preformed to detect the expression of TLR2/TLR4 in normal canal skin, mucosa and granulation tissue of choronic suppurative otitis media, mucosa, granulation tissue, cholesteatoma epithelium of cholesteatoma, and the differential expression were ananlyzed. RESULTS: (1) the mRNA and protein expression of TLR2 and TLR4 were detected in all normal canal skin, mucosa and granulation tissue of choronic suppurative otitis media, mucosa, granulation tissue, cholesteatoma epithelium of cholesteatoma. (2) Both mRNA and protein level of TLR2/TLR4 in mucosa of chronic suppurative otitis media and cholesteatoma were higher than those in normal canal skin, but lower in cholesteatoma epithelium, there was no significant difference in mucosa of the two otitis media groups. (3) The mRNA and protein expressiom of TLR2/TLR4 in granulation tissue of chronic suppurative otitis media and cholesteatoma were significant increased when compared with normal canal skin, and TLR2 expression level was higher in granulation tissue of cholesteatoma than in choronic suppurative otitis meida. (4) TLR2/TLR4 positive cells mainly infiltrated in granulations, significantly more than in normal skin, while fewer in the epithelium of cholesteatoma. CONCLUSIONS: Differential expression of TLR2 and TLR4 in mucosa suggests middle ear is a TLR2/TLR4 participated functional modulation of the innate immune system and also suggests that they may play a different role in the pathophysiology of chronic otitis media and cholesteatoma.



RGD Manual Disease Annotations    Click to see Annotation Detail View

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
TLR2Humancholesteatoma  IEP mRNA and protein:increased expression:mucosa:RGD 
TLR4Humancholesteatoma  IEP mRNA and protein:increased expression:mucosa:RGD 
Tlr2Mousecholesteatoma  ISOTLR2 (Homo sapiens)mRNA and protein:increased expression:mucosa:RGD 
Tlr2Ratcholesteatoma  ISOTLR2 (Homo sapiens)mRNA and protein:increased expression:mucosa:RGD 
Tlr4Ratcholesteatoma  ISOTLR4 (Homo sapiens)mRNA and protein:increased expression:mucosa:RGD 
Tlr4Mousecholesteatoma  ISOTLR4 (Homo sapiens)mRNA and protein:increased expression:mucosa:RGD 
TLR2Humansuppurative otitis media  IEP mRNA and protein:increased expression:mucosa:RGD 
TLR4Humansuppurative otitis media  IEP mRNA and protein:increased expression:mucosa:RGD 
Tlr2Ratsuppurative otitis media  ISOTLR2 (Homo sapiens)mRNA and protein:increased expression:mucosa:RGD 
Tlr2Mousesuppurative otitis media  ISOTLR2 (Homo sapiens)mRNA and protein:increased expression:mucosa:RGD 
Tlr4Ratsuppurative otitis media  ISOTLR4 (Homo sapiens)mRNA and protein:increased expression:mucosa:RGD 
Tlr4Mousesuppurative otitis media  ISOTLR4 (Homo sapiens)mRNA and protein:increased expression:mucosa:RGD 

Objects Annotated

Genes (Rattus norvegicus)
Tlr2  (toll-like receptor 2)
Tlr4  (toll-like receptor 4)

Genes (Mus musculus)
Tlr2  (toll-like receptor 2)
Tlr4  (toll-like receptor 4)

Genes (Homo sapiens)
TLR2  (toll like receptor 2)
TLR4  (toll like receptor 4)


Additional Information