RGD Reference Report - [The expression of matrix metalloproteinase-1 and interleukin-1-alpha messenger ribonucleic acid in human middle ear cholesteatoma]. - Rat Genome Database

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[The expression of matrix metalloproteinase-1 and interleukin-1-alpha messenger ribonucleic acid in human middle ear cholesteatoma].

Authors: Liu, J  Lin, G  Huang, J  Jiang, Z 
Citation: Liu J, etal., Zhonghua Er Bi Yan Hou Ke Za Zhi. 2002 Feb;37(1):30-3.
RGD ID: 7794712
Pubmed: (View Article at PubMed) PMID:12768791

OBJECTIVE: To explore the expression of matrix metalloproteinase-1 (MMP1), tissue inhibitor of metalloproteinase-1(TIMP1) and interleukin-1-alpha messenger ribonucleic acid(IL-1 alpha mRNA) in acquired middle ear cholesteatoma, and to evaluate their roles in the molecular mechanisms of bone resorption. METHODS: Tissue specimens from 32 cases of acquired middle ear cholesteatoma and 14 cases of external ear skin were examined by immunohistochemical S-P method for MMP1 and TIMP1, and by in situ hybridization for IL-1 alpha mRNA. RESULTS: All 32 samples of cholesteatoma showed a stronger expression of MMP1 than the external ear skin. The integral absorbency of MMP1 in the two types of tissues were 2,018.26 +/- 174.89 and 1,428.35 +/- 123.39, respectively, with statistically significant difference between them. Neither of the two types of epithelial cells showed a remarkable expression of TIMP1. The cells hybridized for the antisence probes IL-1 alpha mRNA were only confined to the basal layer; in cholesteatoma, besides the basal cell layers, keratinocytes of the suprabasal cell layers were also found to contain specific hybridizations. The level of IL-1 alpha mRNA measured in cholesteatoma was significantly higher than that in the external ear skin. CONCLUSION: The overexpression of MMP1 and a derailment of the normally controlled MMPs-TIMPs system could play an active role in the molecular mechanisms of cholesteatoma invasion into the bone. The upregulation of IL-1 alpha might contribute to the increasing secretions of MMP1 in cholesteatoma.

Annotation

Disease Annotations    

Objects Annotated

Genes (Rattus norvegicus)
Il1a  (interleukin 1 alpha)
Mmp1  (matrix metallopeptidase 1)

Genes (Mus musculus)
Il1a  (interleukin 1 alpha)
Mmp1a  (matrix metallopeptidase 1a (interstitial collagenase))

Genes (Homo sapiens)
IL1A  (interleukin 1 alpha)
MMP1  (matrix metallopeptidase 1)


Additional Information