RGD Reference Report - Species-specific activity of rat recombinant interleukin-1 beta. - Rat Genome Database

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Species-specific activity of rat recombinant interleukin-1 beta.

Authors: Liu, C  Bai, Y  Ganea, D  Hart, RP 
Citation: Liu C, etal., J Interferon Cytokine Res. 1995 Nov;15(11):985-92.
RGD ID: 7401228
Pubmed: PMID:8590311   (View Abstract at PubMed)
DOI: DOI:10.1089/jir.1995.15.985   (Journal Full-text)

We have constructed expression clones for mature rat interleukin-1 beta (IL-1 beta). Using these clones, rat recombinant IL-1 beta (rrIL-1 beta) was prepared and purified by a single-step cation-exchange chromatography method. In addition, synthetic rrIL-1 beta mRNA was transcribed and used to synthesize radiolabeled 35S-rrIL-1 beta for binding studies. The rrIL-1 beta produced was demonstrated to have IL-1 bioactivity in a mouse thymocyte proliferation assay, in which it could be blocked by inclusion of human recombinant IL-1 receptor antagonist (hrIL-1Ra). Similarly, rrIL-1 beta induced LIF mRNA expression in cultured rat sympathetic ganglia. In each case, the maximal dose of rrIL-1 beta was similar to that of human IL-1 beta. However, the peak response in rat tissue was twofold higher with rat IL-1 beta than with human IL-1 beta. The expression of rrIL-1 beta provides a useful tool for studying IL-1 receptors in rat, in which human and mouse IL-1 beta have been found to bind poorly. Species-specific activity of rat IL-1 beta may correlate with compensatory ionic properties of rat IL-1 receptor.



Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Il1bRatpositive regulation of immature T cell proliferation in thymus  IDA  RGD 

Objects Annotated

Genes (Rattus norvegicus)
Il1b  (interleukin 1 beta)


Additional Information