RGD Reference Report - Synergistic uveitic effects of tumor necrosis factor-alpha and interleukin-1 beta. - Rat Genome Database

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Synergistic uveitic effects of tumor necrosis factor-alpha and interleukin-1 beta.

Authors: Fleisher, LN  Ferrell, JB  McGahan, MC 
Citation: Fleisher LN, etal., Invest Ophthalmol Vis Sci. 1992 Jun;33(7):2120-7.
RGD ID: 7401178
Pubmed: PMID:1318867   (View Abstract at PubMed)

Tumor necrosis factor (TNF) and interleukin-1 (IL-1), cytokines with multiple, overlapping biologic activities, have been shown to interact synergistically in nonocular tissues. To test the hypothesis that coinjection of TNF and IL-1 interact synergistically in the eye, low, marginally inflammatory doses of human recombinant TNF-alpha (4000 U), IL-1 beta (40 U), and TNF-alpha+IL-1 beta (TNF-alpha/IL-1 beta) were injected into the vitreal chamber of the rabbit eye, and inflammation was assessed at 6, 24, 48, and 168 hr post-cytokine injection. TNF-alpha/IL-1 beta induced an anterior uveitis that was barely detectable at 6 hr, increased at 24 hr, peaked at 48 hr, and largely resolved by 168 hr. Synergy was observed for infiltration of inflammatory leukocytes into aqueous humor at 24 and 48 hr and for protein and prostaglandin E levels in aqueous humor at 48 hr. Based upon protein levels in vitreous humor, TNF-alpha/IL-1 beta also induced a posterior uveitis. This posterior uveitis was not apparent until 48 hr and then increased significantly at 168 hr. At 48 and 168 hr, the effects of TNF-alpha/IL-1 beta on protein levels in vitreous humor were consistent with a synergistic interaction. Results of separate experiments using higher dose combinations of TNF-alpha/IL-1 beta and a longer time course suggested that the effects of TNF-alpha/IL-1 beta on the blood vitreous barrier persisted beyond 168 hr. The results of this study support the hypothesis that TNF-alpha and IL-1 beta interact synergistically when injected into the rabbit eye.(ABSTRACT TRUNCATED AT 250 WORDS)



RGD Manual Disease Annotations    Click to see Annotation Detail View

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
IL1BHumanuveitis  IDA  RGD 
Il1bRatuveitis  ISOIL1B (Homo sapiens) RGD 
Il1bMouseuveitis  ISOIL1B (Homo sapiens) RGD 
TNFHumanuveitis  IDA  RGD 
TnfRatuveitis  ISOTNF (Homo sapiens) RGD 
TnfMouseuveitis  ISOTNF (Homo sapiens) RGD 

Objects Annotated

Genes (Rattus norvegicus)
Il1b  (interleukin 1 beta)
Tnf  (tumor necrosis factor)

Genes (Mus musculus)
Il1b  (interleukin 1 beta)
Tnf  (tumor necrosis factor)

Genes (Homo sapiens)
IL1B  (interleukin 1 beta)
TNF  (tumor necrosis factor)


Additional Information