RGD Reference Report - Polymorphisms of the glucocorticoid receptor gene in laboratory and wild rats: steroid binding properties of trinucleotide CAG repeat length variants. - Rat Genome Database

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Polymorphisms of the glucocorticoid receptor gene in laboratory and wild rats: steroid binding properties of trinucleotide CAG repeat length variants.

Authors: Heeley, RP  Gill, E  Van Zutphen, B  Kenyon, CJ  Sutcliffe, RG 
Citation: Heeley RP, etal., Mamm Genome 1998 Mar;9(3):198-203.
RGD ID: 737877
Pubmed: (View Article at PubMed) PMID:9501302

The polyglutamine tract, beginning at codon 75 in the N-terminal modulatory domain of rat glucocorticoid receptor (rGR), was analyzed in 61 inbred strains and 155 wild caught Rattus norvegicus. A discontinuous distribution of repeat lengths was found (7, 17-23 repeats). To investigate the possible significance of this distribution, full-length rGR cDNAs with 7, 18, 20, and 21 CAG repeats were expressed in CV-1 cells, and the resulting GR protein analyzed by Western blots and extensive Scatchard analyses. The quantity and steroid binding capacity of GR, together with the binding affinities for dexamethasone and corticosterone, were found to be indistinguishable for the four repeat alleles. From the sequencing of four inbred strains CAG repeat variants were found to be flanked by silent allelic substitutions at nucleotide positions 198, 531, and 711. The four variable sites extended over 471-519 bp of coding sequence, forming six Grl haplotypes. The results are discussed in the light of genetic studies on the Milan hypertensive and normotensive strains of rat. Codon sequence of rat GR required amendment at the following residues: D98, G226, D260, P600, and F602.

Annotation

Objects referenced in this article
Strain A2/Colle null Rattus norvegicus
Strain ACI/Kun null Rattus norvegicus
Strain ACI/SegHsd null Rattus norvegicus
Strain ACI/Ztm null Rattus norvegicus
Strain AGUS/OlaHsd null Rattus norvegicus
Strain ALC/Colle null Rattus norvegicus
Strain AMORAT/Wsl null Rattus norvegicus
Strain AO/OlaHsd null Rattus norvegicus
Strain ARISTORAT/Wsl null Rattus norvegicus
Strain AS/Ztm null Rattus norvegicus
Strain AUG/OlaHsd null Rattus norvegicus
Strain BBWB/Mol null Rattus norvegicus
Strain BDII/Ztm null Rattus norvegicus
Strain BDIV/lfz null Rattus norvegicus
Strain BDX/Cub null Rattus norvegicus
Strain BN/Crl null Rattus norvegicus
Strain BN/Cub null Rattus norvegicus
Strain BN/Gro null Rattus norvegicus
Strain BN/Gut null Rattus norvegicus
Strain BN/Han null Rattus norvegicus
Strain BN/Maas null Rattus norvegicus
Strain BN/Mol null Rattus norvegicus
Strain BN/NHsdCpb null Rattus norvegicus
Strain BN/OlaHsd null Rattus norvegicus
Strain BN/Orl null Rattus norvegicus
Strain BN/RijKun null Rattus norvegicus
Strain BN/RijN null Rattus norvegicus
Strain BN/SsN null Rattus norvegicus
Strain BP/Cub null Rattus norvegicus
Strain BS/Ztm null Rattus norvegicus
Strain BUF/Ztm null Rattus norvegicus
Strain CAP/Kuv null Rattus norvegicus
Strain COP/OlaHsd null Rattus norvegicus
Strain DA/Ztm null Rattus norvegicus
Strain DZB/Gro null Rattus norvegicus
Strain E3/Ztm null Rattus norvegicus
Strain F344/Ztm null Rattus norvegicus
Strain GC/Kun null Rattus norvegicus
Strain LE/Ztm null Rattus norvegicus
Strain LEP/Cub null Rattus norvegicus
Strain LEW/Cub null Rattus norvegicus
Strain LEW/Gut null Rattus norvegicus
Strain LEW/Han null Rattus norvegicus
Strain LEW/Ipcv null Rattus norvegicus
Strain LEW/Kuv null Rattus norvegicus
Strain LEW/Maas null Rattus norvegicus
Strain LEW/Nhg null Rattus norvegicus
Strain LEW/NHsdCpb null Rattus norvegicus
Strain LEW/Orl null Rattus norvegicus
Strain LEW/SsNHsd null Rattus norvegicus
Strain LEW/Ztm LEW/Ztm Rattus norvegicus
Strain LH/Ztu null Rattus norvegicus
Strain LOU/CHan Louvain Rattus norvegicus
Strain MNS/Gib Milan Normotensive Strain Rattus norvegicus
Strain MWF/Hsd Munich Wistar Fromter Rattus norvegicus
Strain NAR/SaU non-albumin rat Rattus norvegicus
Strain OM/Ztm Osborne-Mendel Rattus norvegicus
Strain PAR/Wsl null Rattus norvegicus
Strain PVG/OlaHsd null Rattus norvegicus
Strain R/A null Rattus norvegicus
Strain R/AEurRij null Rattus norvegicus
Strain R/AWa null Rattus norvegicus
Strain SD/Rij null Rattus norvegicus
Strain SDL/Ipcv null Rattus norvegicus
Strain SPRD/Ztm null Rattus norvegicus
Strain SR/JrIpcv null Rattus norvegicus
Strain SR/JrMol null Rattus norvegicus
Strain SS/JrIpcv null Rattus norvegicus
Strain U/A null Rattus norvegicus
Strain WF/Gut null Rattus norvegicus
Strain WF/Mol null Rattus norvegicus
Strain WF/NHsd null Rattus norvegicus
Strain WF/Ztm null Rattus norvegicus
Strain WIST/Nhg Wistar Rattus norvegicus
Strain WKY/Ztm null Rattus norvegicus
Strain WOKA/K null Rattus norvegicus

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