RGD Reference Report - Molecular cloning and expression of a rat prostaglandin E2 receptor of the EP2 subtype. - Rat Genome Database

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Molecular cloning and expression of a rat prostaglandin E2 receptor of the EP2 subtype.

Authors: Nemoto, K  Pilbeam, CC  Bilak, SR  Raisz, LG 
Citation: Nemoto K, etal., Prostaglandins 1997 Oct;54(4):713-25.
RGD ID: 737645
Pubmed: PMID:9440134   (View Abstract at PubMed)

Prostaglandin E2 (PGE2) is a potent local mediator of cell growth and differentiation in various tissues. The receptors for PGE2 have been classified into four pharmacological subtypes, EP1, EP2, EP3, and EP4, based on the responses to selective agonists and antagonists. We have cloned a functional cDNA for the rat EP2 receptor subtype from a rat lung cDNA library. The rat EP2 receptor cDNA encodes 357 amino acid residues having high homology with the human and mouse EP2 receptors and containing seven putative transmembrane domains. In COS-7 cells transfected with rat EP2 cDNA, specific [3H]PGE2 binding was found with a dissociation constant of 14.9 nM, and this binding was inhibited by unlabeled PGE2 and PGE2 alpha. PGE2 and butaprost, an EP2 selective agonist, were effective in increasing the cAMP level in the COS-7 cell transfectants. Northern blot and RT-PCR analysis showed widespread distribution of the EP2 receptor in various tissues. Higher EP2 expression was found in fetal long bones and calvariae than in adult by RT-PCR and in situ hybridization, suggesting a role for this receptor in rapidly growing skeletal tissue.



Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Ptger2Ratskeletal system development  NAS  RGD 

Molecular Function

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Ptger2Ratprostaglandin E receptor activity  IDA  RGD 

Objects Annotated

Genes (Rattus norvegicus)
Ptger2  (prostaglandin E receptor 2)


Additional Information