RGD Reference Report - Moderate Hypothermia Inhibits Brain Inflammation and Attenuates Stroke-Induced Immunodepression in Rats. - Rat Genome Database

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Moderate Hypothermia Inhibits Brain Inflammation and Attenuates Stroke-Induced Immunodepression in Rats.

Authors: Gu, LJ  Xiong, XX  Ito, T  Lee, J  Xu, BH  Krams, S  Steinberg, GK  Zhao, H 
Citation: Gu LJ, etal., CNS Neurosci Ther. 2013 Aug 24. doi: 10.1111/cns.12160.
RGD ID: 7364869
Pubmed: PMID:23981596   (View Abstract at PubMed)
PMCID: PMC3867545   (View Article at PubMed Central)
DOI: DOI:10.1111/cns.12160   (Journal Full-text)

AIMS: Stroke causes both brain inflammation and immunodepression. Mild-to-moderate hypothermia is known to attenuate brain inflammation, but its role in stroke-induced immunodepression (SIID) of the peripheral immune system remains unknown. This study investigated the effects in rats of moderate intra-ischemic hypothermia on SIID and brain inflammation. METHODS: Stroke was induced in rats by permanent distal middle cerebral artery occlusion combined with transient bilateral common carotid artery occlusion, while body temperature was reduced to 30 degrees C. Real-time PCR, flow cytometry, in vitro T-cell proliferation assays, in vivo delayed-type hypersensitivity (DTH) reaction and confocal microscopy were used to study SIID and brain inflammation. RESULTS: Brief intra-ischemic hypothermia helped maintain certain leukocytes in the peripheral blood and spleen and enhanced T-cell proliferation in vitro and delayed-type hypersensitivity in vivo, suggesting that hypothermia reduces SIID. In contrast, in the brain, brief intra-Ischemic hypothermia inhibited mRNA expression of anti-inflammatory cytokine IL-10 and proinflammatory mediators INF-gamma, TNF-alpha, IL-2, IL-1beta and MIP-2. Brief intra-Ischemic hypothermia also attenuated the infiltration of lymphocytes, neutrophils (MPO+ cells) and macrophages (CD68+ cells) into the ischemic brain, suggesting that hypothermia inhibited brain inflammation. CONCLUSIONS: Brief intra-ischemic hypothermia attenuated SIID and protected against acute brain inflammation.



RGD Manual Disease Annotations    Click to see Annotation Detail View

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
IL10HumanStroke  ISOIl10 (Rattus norvegicus)mRNA:increased expression:brainRGD 
Il10RatStroke  IEP mRNA:increased expression:brainRGD 
Il10MouseStroke  ISOIl10 (Rattus norvegicus)mRNA:increased expression:brainRGD 

Objects Annotated

Genes (Rattus norvegicus)
Il10  (interleukin 10)

Genes (Mus musculus)
Il10  (interleukin 10)

Genes (Homo sapiens)
IL10  (interleukin 10)


Additional Information