RGD Reference Report - Expression profiles of regulatory and helper T-cell-associated genes in nasal polyposis. - Rat Genome Database

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Expression profiles of regulatory and helper T-cell-associated genes in nasal polyposis.

Authors: Li, CW  Zhang, KK  Li, TY  Lin, ZB  Li, YY  Curotto de Lafaille, MA  Shi, L  Wang, DY 
Citation: Li CW, etal., Allergy. 2012 Jun;67(6):732-40. doi: 10.1111/j.1398-9995.2012.02811.x. Epub 2012 Mar 30.
RGD ID: 7364835
Pubmed: PMID:22462754   (View Abstract at PubMed)
DOI: DOI:10.1111/j.1398-9995.2012.02811.x   (Journal Full-text)

BACKGROUND: Nasal polyposis (NP) is a Th2-skewed inflammatory disorder, but it is unclear what role regulatory T cells (T-reg) play in disease pathology. We investigated the expression profiles of T-reg and T-helper-cell-associated genes and their response to glucocorticosteroid (GC) treatment in Chinese patients with NP. METHODS: Biopsies were obtained from 29 non-treated NP patients for comparison with inferior turbinates collected from healthy controls. In 13 patients, NP samples were collected both before and after short-term oral GC treatment. Levels of mRNA for T-cell markers were determined by microarray and quantitative PCR. Cellular infiltrates were assessed by histo- and immunohistochemistry. RESULTS: FOXP3(+) T-reg were increased in GC-naive NP, and numbers were negatively correlated with eosinophil infiltration. Helios staining was not detected, suggesting that FOXP3(+) cells in NP are not thymus-derived T-reg. Compared with controls, mRNA levels corresponding to T-reg genes were significantly increased in NP (FOXP3, TGFB1, IL10, SMAD3, IL2RA, and JAK3), but transcription factors associated with Th2 (GATA3) or Th17 responses (RORc) were significantly reduced. FOXP3 mRNA levels positively correlated with other T-reg cell markers. Microarray analysis showed that most Th2-related markers (e.g., Eotaxin-1, CCL13, and CCL18) were upregulated in GC-naive NP vs controls. GC therapy significantly suppressed eosinophilic inflammation in NP, but did not significantly alter the expression levels of T-reg/Th2-associated genes. CONCLUSIONS: Upregulation of FOXP3(+) -inducible T-reg cells and downregulation of Th2 and Th17 markers in NP indicate a regulatory response occurring at a site of persistent mucosal inflammation. However, immune regulation fails to control the underlying tissue pathology. Expression of T-reg/Th2 markers after GC treatment was unaltered, suggesting that T-cell-driving NP inflammatory mediators are GC resistant.

RGD Manual Disease Annotations    Click to see Annotation Detail View
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
Nasal Polyps  IEP 7364835 RGD 
Nasal Polyps  ISOIL10 (Homo sapiens)7364835; 7364835 RGD 

Objects Annotated

Genes (Rattus norvegicus)
Il10  (interleukin 10)

Genes (Mus musculus)
Il10  (interleukin 10)

Genes (Homo sapiens)
IL10  (interleukin 10)


Additional Information