RGD Reference Report - Cellular uptake and activity of heparin functionalised cerium oxide nanoparticles in monocytes. - Rat Genome Database
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Cellular uptake and activity of heparin functionalised cerium oxide nanoparticles in monocytes.

Authors: Ting, SR  Whitelock, JM  Tomic, R  Gunawan, C  Teoh, WY  Amal, R  Lord, MS 
Citation: Ting SR, etal., Biomaterials. 2013 Jun;34(17):4377-86. doi: 10.1016/j.biomaterials.2013.02.042. Epub 2013 Mar 7.
RGD ID: 7364779
Pubmed: (View Article at PubMed) PMID:23478040
DOI: Full-text: DOI:10.1016/j.biomaterials.2013.02.042

Cerium oxide nanoparticles (nanoceria) are effective in scavenging intracellular reactive oxygen species (ROS). In this study nanoceria synthesized by flame spray pyrolysis (dXRD = 12 nm) were functionalised with heparin via an organosilane linker, 3-aminopropyltriethoxysilane. Nanoceria were functionalised with approximately 130 heparin molecules per nanoparticle as determined by thermo gravimetric analysis. Heparin functionalised nanoceria were more effectively internalised by the human monocyte cell line, U937, and U937 cells that had been activated with phorbol 12 myristate 13-acetate (PMA) than bare nanoceria. The heparin functionalised nanoceria were also more effective in scavenging ROS than nanoceria in both activated and unactivated U937 cells. Heparin coupled nanoceria were found to be biologically active due to their ability to bind fibroblast growth factor 2 and signal through FGF receptor 1. Additionally, the heparin-coupled nanoceria, once internalised by the cells, were found to be degraded by 48 h. Together these data demonstrated that heparin enhanced the biological properties of nanoceria in terms of cellular uptake and ROS scavenging, while the nanoceria themselves were more effective at delivering heparin intracellularly than exposing cells to heparin in solution.

Annotation

Molecular Pathway Annotations    
Objects Annotated

Genes (Rattus norvegicus)
Fgf2  (fibroblast growth factor 2)
Fgfr1  (Fibroblast growth factor receptor 1)

Genes (Mus musculus)
Fgf2  (fibroblast growth factor 2)
Fgfr1  (fibroblast growth factor receptor 1)

Genes (Homo sapiens)
FGF2  (fibroblast growth factor 2)
FGFR1  (fibroblast growth factor receptor 1)


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