RGD Reference Report - Sustained inhibition of corneal neovascularization by genetic ablation of CCR5. - Rat Genome Database

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Sustained inhibition of corneal neovascularization by genetic ablation of CCR5.

Authors: Ambati, BK  Anand, A  Joussen, AM  Kuziel, WA  Adamis, AP  Ambati, J 
Citation: Ambati BK, etal., Invest Ophthalmol Vis Sci 2003 Feb;44(2):590-3.
RGD ID: 734790
Pubmed: (View Article at PubMed) PMID:12556387

PURPOSE: To determine whether genetic ablation of the CC chemokine receptor CCR5 (involved in leukocyte and endothelial chemotaxis) inhibits the development of corneal neovascularization. METHODS: Wild-type C57BL/6J mice and species-specific counterparts with targeted homozygous disruption of the CCR5 gene underwent chemical and mechanical denudation of corneal and limbal epithelium. Corneas were harvested 2 and 4 weeks after injury. Neovascularization was quantified by CD31 immunostaining. Expression of VEGF protein was quantified by ELISA. RESULTS: The mean percentages of neovascularized corneal area in control mice and CCR5-deficient mice 2 weeks after denudation were 58.3% and 38.5% (P = 0.05), respectively. At 4 weeks after denudation, the corresponding percentages were 67.6% and 44.0% (P = 0.028). In CCR5-deficient mice, VEGF protein levels were reduced 51.1% at 2 weeks (P = 0.05) after injury and 37.3% at 4 weeks (P = 0.03). CONCLUSIONS: CCR5-deficient mice showed a persistent 34% to 35% inhibition of corneal neovascularization for up to 4 weeks. This inhibition correlates with reduced expression of VEGF. These data implicate CCR5 as one essential component in the development of corneal neovascularization.

Annotation

Disease Annotations    

Objects Annotated

Genes (Rattus norvegicus)
Ccr5  (C-C motif chemokine receptor 5)

Genes (Mus musculus)
Ccr5  (chemokine (C-C motif) receptor 5)

Genes (Homo sapiens)
CCR5  (C-C motif chemokine receptor 5)


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