RGD Reference Report - Arachidonate 5-lipoxygenase promoter genotype, dietary arachidonic acid, and atherosclerosis. - Rat Genome Database

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Arachidonate 5-lipoxygenase promoter genotype, dietary arachidonic acid, and atherosclerosis.

Authors: Dwyer, JH  Allayee, H  Dwyer, KM  Fan, J  Wu, H  Mar, R  Lusis, AJ  Mehrabian, M 
Citation: Dwyer JH, etal., N Engl J Med 2004 Jan 1;350(1):29-37.
RGD ID: 734559
Pubmed: PMID:14702425   (View Abstract at PubMed)
DOI: DOI:10.1056/NEJMoa025079   (Journal Full-text)

BACKGROUND: Leukotrienes are inflammatory mediators generated from arachidonic acid (polyunsaturated n-6 fatty acid) by the enzyme 5-lipoxygenase. Since atherosclerosis involves arterial inflammation, we hypothesized that a polymorphism in the 5-lipoxygenase gene promoter could relate to atherosclerosis in humans and that this effect could interact with the dietary intake of competing 5-lipoxygenase substrates. METHODS: We determined 5-lipoxygenase genotypes, carotid-artery intima-media thickness, and markers of inflammation in a randomly sampled cohort of 470 healthy, middle-aged women and men from the Los Angeles Atherosclerosis Study. Dietary arachidonic acid and marine n-3 fatty acids (including a competing 5-lipoxygenase substrate that reduces the production of inflammatory leukotrienes) were measured with the use of six 24-hour recalls of food intake. RESULTS: Variant 5-lipoxygenase genotypes (lacking the common allele) were found in 6.0 percent of the cohort. Mean (+/-SE) intima-media thickness adjusted for age, sex, height, and racial or ethnic group was increased by 80+/-19 microm (95 percent confidence interval, 43 to 116; P<0.001) among carriers of two variant alleles, as compared with carriers of the common (wild-type) allele. In multivariate analysis, the increase in intima-media thickness among carriers of two variant alleles (62 microm, P<0.001) was similar in this cohort to that associated with diabetes (64 microm, P=0.01), the strongest common cardiovascular risk factor. Increased dietary arachidonic acid significantly enhanced the apparent atherogenic effect of genotype, whereas increased dietary intake of n-3 fatty acids blunted the effect. Finally, the plasma level of C-reactive protein, a marker of inflammation, was increased by a factor of 2 among carriers of two variant alleles as compared with that among carriers of the common allele. CONCLUSIONS: Variant 5-lipoxygenase genotypes identify a subpopulation with increased atherosclerosis. The observed diet-gene interactions further suggest that dietary n-6 polyunsaturated fatty acids promote, whereas marine n-3 fatty acids inhibit, leukotriene-mediated inflammation that leads to atherosclerosis in this subpopulation.

RGD Manual Disease Annotations    Click to see Annotation Detail View
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
arteriosclerosis  IAGP 734559 RGD 
arteriosclerosis  TAS 734559 RGD 
arteriosclerosis  ISOALOX5 (Homo sapiens)734559; 734559 RGD 

Objects Annotated

Genes (Rattus norvegicus)
Alox5  (arachidonate 5-lipoxygenase)

Genes (Mus musculus)
Alox5  (arachidonate 5-lipoxygenase)

Genes (Homo sapiens)
ALOX5  (arachidonate 5-lipoxygenase)


Additional Information