Genetics of behaviour: phenotypic and molecular study of rats derived from high- and low-alcohol consuming lines.
Terenina-Rigaldie, E Moisan, MP Colas, A Beauge, F Shah, KV Jones, BC Mormede, P
||Terenina-Rigaldie E, etal., Pharmacogenetics 2003 Sep;13(9):543-54.
||(View Article at PubMed) PMID:12972953
The aim of this study was to analyse the genetic basis of excessive ethanol consumption and its relationship with emotional reactivity. The high-ethanol preferring line of rats used is characterized by a: (i) high voluntary consumption of alcohol; (ii) high sensitivity to taste reinforcement (saccharine, quinine); (iii) high locomotor activity in a novel environment; and (iv) low emotional reactivity, these features being opposite in the Wistar-Kyoto (WKY) rat strain. The F2 population demonstrated a very large variability in these behavioural traits, and factor analysis revealed that these characteristics appear to be largely unrelated to each other. The molecular bases for these differences were investigated by quantitative trait loci (QTL) analysis. For this purpose, the 196 F2 rats were genotyped with regularly distributed markers on the whole genome, and genetic linkage maps were generated for all subsequent QTL analyses. A locus with a maximum LOD score of 7.6 and accounting for approximately 61% of the genetic variance of the trait in the F2 population was detected on chromosome 4 for alcohol drinking. In the same region, we found a QTL related to the reinforcement properties of saccharin, with a significant LOD score of 4.9 and explaining 46% of the variance of the trait. Other significant QTL were found for plus maze behaviour and open field activity on chromosome 1. Current research aims to identify the gene(s) involved.
Phenotype Values via PhenoMiner
|Alc14 (Alcohol consumption QTL 14)|
|Alc15 (Alcohol consumption QTL 15)|
|Alc16 (Alcohol consumption QTL 16)|
|Alc17 (Alcohol consumption QTL 17)|
|Anxrr12 (Anxiety related response QTL 12)|
|Anxrr13 (Anxiety related response QTL 13)|
|Anxrr14 (Anxiety related response QTL 14)|
|Anxrr15 (Anxiety related response QTL 15)|
|Sach4 (Saccharine consumption QTL 4)|
|Sach5 (Saccharine consumption QTL 5)|
|HEP (high ethanol preferring)|