RGD Reference Report - Cloning and sequence analysis of cDNA for rat liver uricase. - Rat Genome Database

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Cloning and sequence analysis of cDNA for rat liver uricase.

Authors: Motojima, K  Kanaya, S  Goto, S 
Citation: Motojima K, etal., J Biol Chem 1988 Nov 15;263(32):16677-81.
RGD ID: 730101
Pubmed: PMID:3182808   (View Abstract at PubMed)

We have isolated cDNA clones for rat liver uricase using an oligonucleotide corresponding to the N-terminal sequence of 8 amino acids. The nucleotide sequences of the cDNAs have been determined, and the amino acid sequence of the protein deduced. A 867-base open reading frame coding for 289 amino acids, corresponding to a molecular mass of 33,274 daltons, was confirmed by matching eight sequences of a total of 53 amino acids from peptide sequence analyses of the fragments generated by lysyl endopeptidase digestion of purified rat liver uricase. The deduced amino acid sequence of rat liver uricase shares 40% homology with that of soybean nodulin-specific uricase and has an N-terminal extension of 7 amino acids. In contrast, soybean uricase has a C-terminal extension of 12 amino acids, which is presumably the result of local gene duplication. Completely different N- and C-terminal structures of the two uricases suggest that the signals for targeting the proteins to the peroxisome are not located on the terminal continuous stretches of amino acids.



Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
UoxRatpurine nucleobase catabolic process  TAS  RGD 

Cellular Component

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
UoxRatperoxisomal matrix  TAS  RGD 

Molecular Function

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
UoxRaturate oxidase activity  TAS  RGD 

Objects Annotated

Genes (Rattus norvegicus)
Uox  (urate oxidase)


Additional Information