RGD Reference Report - Hepatic thrombopoietin mRNA is increased in acute inflammation. - Rat Genome Database

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Hepatic thrombopoietin mRNA is increased in acute inflammation.

Authors: Wolber, EM  Fandrey, J  Frackowski, U  Jelkmann, W 
Citation: Wolber EM, etal., Thromb Haemost 2001 Dec;86(6):1421-4.
RGD ID: 730050
Pubmed: PMID:11776309   (View Abstract at PubMed)

The plasma concentration of thrombopoietin (TPO) in general is inversely related to the mass of platelets and megakaryocytes. However, reactive thrombocytosis of inflammatory disease is accompanied by elevated TPO levels. To investigate whether the rate of TPO mRNA expression is altered during acute inflammation, rats were injected with bacterial lipopolysaccharide (LPS). After 6 h, total RNA from liver and kidney was reverse transcribed and analyzed by competitive PCR for TPO and glyceraldehyde-3-phosphate dehydrogenase (GAPDH). LPS-treated rats showed a significant increase in hepatic TPO mRNA concentration. The ratio of TPO to GAPDH mRNA was 3.5 +/- 0.6% in the livers of control rats and 8.3 +/- 2.0% in the livers of LPS-treated rats (mean +/- SD). Thus, reactive thrombocytosis of inflammatory disease might result from an increase in hepatic TPO production. Since platelets are involved in the immune reaction, reactive thrombocytosis may be a mechanism of host defense.

Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
response to lipopolysaccharide  IEP 730050 RGD 

Cellular Component
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
extracellular region  IDA 730050 RGD 

Objects Annotated

Genes (Rattus norvegicus)
Thpo  (thrombopoietin)

Objects referenced in this article
Gene THPO thrombopoietin Homo sapiens
Gene Thpo thrombopoietin Mus musculus

Additional Information