RGD Reference Report - Phospholipid binding by a synaptic vesicle protein homologous to the regulatory region of protein kinase C. - Rat Genome Database

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Phospholipid binding by a synaptic vesicle protein homologous to the regulatory region of protein kinase C.

Authors: Perin, MS  Fried, VA  Mignery, GA  Jahn, R  Sudhof, TC 
Citation: Perin MS, etal., Nature 1990 May 17;345(6272):260-3.
RGD ID: 729959
Pubmed: PMID:2333096   (View Abstract at PubMed)
DOI: DOI:10.1038/345260a0   (Journal Full-text)

Neurotransmitters are released at synapses by the Ca2(+)-regulated exocytosis of synaptic vesicles, which are specialized secretory organelles that store high concentrations of neurotransmitters. The rapid Ca2(+)-triggered fusion of synaptic vesicles is presumably mediated by specific proteins that must interact with Ca2+ and the phospholipid bilayer. We now report that the cytoplasmic domain of p65, a synaptic vesicle-specific protein that binds calmodulin contains an internally repeated sequence that is homologous to the regulatory C2-region of protein kinase C (PKC). The cytoplasmic domain of recombinant p65 binds acidic phospholipids with a specificity indicating an interaction of p65 with the hydrophobic core as well as the headgroups of the phospholipids. The binding specificity resembles PKC, except that p65 also binds calmodulin, placing the C2-regions in a context of potential Ca2(+)-regulation that is different from PKC. This is a novel homology between a cellular protein and the regulatory domain of protein kinase C. The structure and properties of p65 suggest that it may have a role in mediating membrane interactions during synaptic vesicle exocytosis.

Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
calcium-ion regulated exocytosis  TAS 729959 RGD 
synaptic vesicle exocytosis  TAS 729959 RGD 

Cellular Component
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
synaptic vesicle membrane  TAS 729959 RGD 

Molecular Function
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
calcium ion binding  TAS 729959 RGD 
calmodulin binding  IDA 729959 RGD 
phospholipid binding  IDA 729959 RGD 

Objects Annotated

Genes (Rattus norvegicus)
Syt1  (synaptotagmin 1)


Additional Information