RGD Reference Report - Expression cloning and characterization of the TGF-beta type III receptor. - Rat Genome Database

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Expression cloning and characterization of the TGF-beta type III receptor.

Authors: Wang, XF  Lin, HY  Ng-Eaton, E  Downward, J  Lodish, HF  Weinberg, RA 
Citation: Wang XF, etal., Cell 1991 Nov 15;67(4):797-805.
RGD ID: 729942
Pubmed: (View Article at PubMed) PMID:1657407

The rat TGF-beta type III receptor cDNA has been cloned by overexpression in COS cells. The encoded receptor is an 853 amino acid protein with a large N-terminal extracellular domain containing at least one site for glycosaminoglycan addition, a single hydrophobic transmembrane domain, and a 41 amino acid cytoplasmic tail with no obvious signaling motif. Introduction of the cDNA into COS cells and L6 myoblasts induces expression of a heterogenously glycosylated 280-330 kd protein characteristic of the type III receptor that binds TGF-beta 1 specifically. In L6 myoblasts lacking the endogenous type III receptor, expression of the recombinant receptor leads to an increase in the amount of ligand bound and cross-linked to surface type II TGF-beta receptors. This indicates that the type III receptor may regulate the ligand-binding ability or surface expression of the type II receptor.

Annotation

Gene Ontology Annotations    

Biological Process

Molecular Function

Objects Annotated

Genes (Rattus norvegicus)
Tgfbr3  (transforming growth factor beta receptor 3)


Additional Information