RGD Reference Report - The gene structure of rat androgen-binding protein: identification of potential regulatory deoxyribonucleic acid elements of a follicle-stimulating hormone-regulated protein. - Rat Genome Database

Send us a Message
Submit Data |  Help |  Video Tutorials |  News |  Publications |  Download |  REST API |  Citing RGD |  Contact   
Search RGD Grant Resources Citing RGD About Us Contact Us
Genes Variants Community Projects QTLs Strains Markers Genome Information Ontologies Cell Lines References Download Submit Data
OntoMate (Literature Search) JBrowse (Genome Browser) Synteny Browser (VCMap) Variant Visualizer Multi-Ontology Enrichment (MOET) Gene-Ortholog Location Finder (GOLF) InterViewer (Protein-Protein Interactions) PhenoMiner (Quatitative Phenotypes) Gene Annotator OLGA (Gene List Generator) AllianceMine GViewer (Genome Viewer)
Aging & Age-Related Disease Cancer & Neoplastic Disease Cardiovascular Disease Coronavirus Disease Developmental Disease Diabetes Hematologic Disease Immune & Inflammatory Disease Infectious Disease Liver Disease Neurological Disease Obesity & Metabolic Syndrome Renal Disease Respiratory Disease Sensory Organ Disease
Find Models   new Genetic Models Autism Models Rat PhenoMiner (Quantitative Phenotypes) Chinchilla PhenoMiner Expected Ranges (Quantitative Phenotype) PhenoMiner Term Comparison Hybrid Rat Diversity Panel Phenotypes Phenotypes in Other Animal Models Animal Husbandry Strain Medical Records Phylogenetics Strain Availability Calendar Rats 101 Submissions Photo Archive
Pathways
Rat Community Forum Directory of Rat Laboratories Video Tutorials News RGD Publications RGD Presentations Archive Nomenclature Guidelines Resource Links Laboratory Resources Employment Resources

The gene structure of rat androgen-binding protein: identification of potential regulatory deoxyribonucleic acid elements of a follicle-stimulating hormone-regulated protein.

Authors: Joseph, DR  Hall, SH  Conti, M  French, FS 
Citation: Joseph DR, etal., Mol Endocrinol 1988 Jan;2(1):3-13.
RGD ID: 729840
Pubmed: PMID:2840566   (View Abstract at PubMed)
DOI: DOI:10.1210/mend-2-1-3   (Journal Full-text)

A genomic clone has been characterized for androgen-binding protein (ABP), a Sertoli cell secretory protein that is regulated by androgens and FSH. A 5.3-kilobase pair Sstl DNA fragment was sequenced and found to contain the entire coding region of the gene, which is divided into 8 exons. The major transcription initiation site in the testis was localized by primer extension with two unique oligomers. In addition, a minor initiation site was identified that appears to originate from another promoter. The gene does not contain a conventional TATA box immediately upstream from the major start site; rather, the sequence TACCTA occurs at residue -24. This sequence has been described functionally as a TATA-like element in the SV40 major late gene. Other potential regulatory elements include a sequence related to the cAMP response element at residue -126 base pair. Using primary Sertoli cell cultures, it was found that (Bu)2cAMP or FSH increases ABP mRNA levels 3-5 fold, with a 2-fold increase in the level of secreted ABP. Southern blot analysis of rat genomic DNA indicated that there is a single gene for ABP in the rat. The existence of one gene supports the idea that sex hormone binding globulin produced by fetal rat liver is coded by the same gene.

Objects referenced in this article
Gene Shbg sex hormone binding globulin Rattus norvegicus

Additional Information