RGD Reference Report - Tissue-specific expression of isoaspartyl protein carboxyl methyltransferase gene in rat brain and testis. - Rat Genome Database

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Tissue-specific expression of isoaspartyl protein carboxyl methyltransferase gene in rat brain and testis.

Authors: Mizobuchi, M  Murao, K  Takeda, R  Kakimoto, Y 
Citation: Mizobuchi M, etal., J Neurochem 1994 Jan;62(1):322-8.
RGD ID: 729481
Pubmed: PMID:8263531   (View Abstract at PubMed)

Isoaspartyl protein carboxyl methyltransferase (PIMT) is widely distributed in mammalian tissues. Using a polymerase chain reaction-generated 124-bp DNA fragment from brain cDNA as a probe, four different sizes (approximately 4.0, 2.5, 1.7, and 1.1 kb) of transcripts were detected with northern blot analysis. They were expressed predominantly in rat brain and testis. The major transcripts were 2.5 and 1.7 kb in the brain and 2.5 and 1.1 kb in the testis. One of the major transcripts specific to the testis (1.1 kb) was determined to study the structural difference of major transcripts in the two tissues. This testicular cDNA had neither the 5' (94 nucleotides) nor the 3' (594 nucleotides) end of previously reported brain cDNA corresponding to 1.7 kb. The mRNA levels and enzyme activities of different regions and developmental changes were examined in the brain. The mRNA levels and enzyme activities were concomitantly high in cerebral cortex and hippocampus. Although they increased rapidly approximately 30 days after birth in the testis and decreased in aged rats, they increased gradually after birth and remained high during the aging of the brain. Both structural and developmental studies show that the expression of the PIMT gene in brain and testis is regulated in a tissue-specific manner.

Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process

Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Pcmt1RatS-adenosylhomocysteine metabolic process  IDA  RGD 
Pcmt1RatS-adenosylmethionine metabolic process  IDA  RGD 

Molecular Function

Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Pcmt1Ratprotein-L-isoaspartate (D-aspartate) O-methyltransferase activity  IDA  RGD 
Pcmt1RatS-adenosylmethionine-dependent methyltransferase activity  IDA  RGD 

Molecular Pathway Annotations    Click to see Annotation Detail View

RGD Manual Annotations

Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
PCMT1Humanhomocysteine metabolic pathway   ISS  RGD 
Pcmt1Rathomocysteine metabolic pathway   IDA  RGD 
PCMT1Humanmethionine cycle/metabolic pathway   ISS  RGD 
Pcmt1Ratmethionine cycle/metabolic pathway   IDA  RGD 
Objects Annotated

Genes (Rattus norvegicus)
Pcmt1  (protein-L-isoaspartate (D-aspartate) O-methyltransferase 1)

Genes (Homo sapiens)
PCMT1  (protein-L-isoaspartate (D-aspartate) O-methyltransferase)

Additional Information