RGD Reference Report - The slow axonal transport of the microtubule-associated protein tau and the transport rates of different isoforms and mutants in cultured neurons. - Rat Genome Database

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The slow axonal transport of the microtubule-associated protein tau and the transport rates of different isoforms and mutants in cultured neurons.

Authors: Utton, MA  Connell, J  Asuni, AA  Van Slegtenhorst, M  Hutton, M  De Silva, R  Lees, AJ  Miller, CC  Anderton, BH 
Citation: Utton MA, etal., J Neurosci 2002 Aug 1;22(15):6394-400.
RGD ID: 729314
Pubmed: PMID:12151518   (View Abstract at PubMed)
PMCID: PMC6758152   (View Article at PubMed Central)
DOI: DOI:20026670   (Journal Full-text)

We demonstrate that the microtubule-associated protein tau, in the form of enhanced green fluorescent protein (EGFP) tau, is transported along axons of neurons in culture in the slow component of axonal transport with a speed comparable with that previously measured in vivo. It was demonstrated that the EGFP tag has no effect on transport characteristics, and the methodology enables slow transport rates of individual tau isoforms and tau mutants to be measured. We also expressed EGFP-tagged tau isoforms containing either three or four C-terminal repeats and zero or two N-terminal inserts in cultured neurons. No significant differences were found in the average rate of slow transport of the wild-type tau isoforms, suggesting that the exon 10 C-terminal repeat or the N-terminal inserts do not contain regions that play a significant regulatory role in axonal transport. Similarly, we found that missense mutations in tau have no noticeable effect on the rate of transport; hence their ability to cause neurodegeneration is by another mechanism other than that affecting the overall slow axonal transport of tau.

Objects referenced in this article
Gene Mapt microtubule-associated protein tau Rattus norvegicus

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