RGD Reference Report - Characterization of a growth hormone-releasing hormone binding site in the rat renal medulla. - Rat Genome Database

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Characterization of a growth hormone-releasing hormone binding site in the rat renal medulla.

Authors: Boulanger, L  Girard, N  Strecko, J  Gaudreau, P 
Citation: Boulanger L, etal., Peptides 2002 Jan;23(1):43-50.
RGD ID: 728748
Pubmed: PMID:11814616   (View Abstract at PubMed)

Receptor binding analysis was performed in the renal medulla from 2-month-old rats, an extrapituitary tissue containing the highest level of GHRH receptor mRNA. At 4 degrees C, in the presence of a cocktail of protease inhibitors, binding of [125I-Tyr(10)]hGHRH NH(2) to medullary homogenates was specific, time-dependent, reversible and saturable (K(d): 28 nM; B(max): 30 fmol/mg prot.). In these experimental conditions, no change of binding parameters could be detected in the course of aging. The structure-affinity profile was different in the two tissues and chemical cross-linking revealed the presence of 65-, 55- and 38-kDa 125I-GHRH-labeled complexes in the renal medulla compared to 65-, 47- and 28-kDa radioactive complexes in the anterior pituitary. It is suggested that GHRH binding sites, and possibly the receptor, may be different in the two tissues.

Gene Ontology Annotations    Click to see Annotation Detail View

Molecular Function
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
growth hormone-releasing hormone receptor activity  IDA 728748 RGD 

Objects Annotated

Genes (Rattus norvegicus)
Ghrhr  (growth hormone releasing hormone receptor)

Objects referenced in this article
Gene Ghrh growth hormone releasing hormone Rattus norvegicus

Additional Information