RGD Reference Report - Cloning and characterization of a single-stranded DNA binding protein that specifically recognizes deoxycytidine stretch. - Rat Genome Database

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Cloning and characterization of a single-stranded DNA binding protein that specifically recognizes deoxycytidine stretch.

Authors: Ito, K  Sato, K  Endo, H 
Citation: Ito K, etal., Nucleic Acids Res 1994 Jan 11;22(1):53-8.
RGD ID: 728600
Pubmed: PMID:8127654   (View Abstract at PubMed)
PMCID: PMC307745   (View Article at PubMed Central)

We previously identified a G-rich silencer element involved in negative regulation of catalase gene expression in some hepatoma cells (Mol. Cell. Biol., (1992), 12, 2525-2533). To study a nuclear binding protein for this element, we screened cDNA libraries from a rat ascites hepatoma cell line by binding with a synthetic oligonucleotide probe and obtained several clones. One of them, designated SW, was studied in detail. A clone (SW2) of this series contained a near full length cDNA encoding a putative peptide with 463 amino acid residues. We isolated this peptide as a fusion protein. It was found that the protein strongly bound to the C-stretch of the DNA sequence in a single strand specific fashion, but absolutely did not to G-rich sequence. The protein bound weakly to the corresponding double-stranded DNA as well as to C-rich RNA sequence. This protein, though not the expected one, was found to be a novel protein whose DNA binding domain was located on the region containing at least 75 amino acid residues of the carboxyl terminus. A proline rich region was also observed in the middle part of the protein. Northern blot profiles indicated extensive and slight expression of both 2.0 kb and 2.7 kb mRNA species in some hepatoma cell lines and in the rat liver, respectively.



Gene Ontology Annotations    Click to see Annotation Detail View

Molecular Function

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
HnrnpkRatC-rich single-stranded DNA binding  IDA  RGD 

Objects Annotated

Genes (Rattus norvegicus)
Hnrnpk  (heterogeneous nuclear ribonucleoprotein K)


Additional Information