RGD Reference Report - Cloning and functional expression of endothelin-converting enzyme from rat endothelial cells. - Rat Genome Database

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Cloning and functional expression of endothelin-converting enzyme from rat endothelial cells.

Authors: Shimada, K  Takahashi, M  Tanzawa, K 
Citation: Shimada K, etal., J Biol Chem 1994 Jul 15;269(28):18275-8.
RGD ID: 728596
Pubmed: PMID:8034569   (View Abstract at PubMed)

Endothelin (ET) is a 21-residue potent vasoconstrictive peptide produced by vascular endothelial cells and formed from its precursor, big endothelin (big ET), by endothelin-converting enzyme (ECE). Here we report the cloning and functional expression of a complementary DNA encoding a rat ECE from endothelial cells. Rat ECE is a highly glycosylated protein consisting of 10 possible N-linked glycosylation sites, a zinc-binding domain, and a single membrane-spanning region. It has structural and sequence homology to neutral endopeptidase and Kell blood group protein, a putative neutral endopeptidase. Monoclonal antibody risen against purified rat lung ECE recognized broad glycosylated bands in membrane fractions prepared from both rat lung and COS cells transfected with a rat ECE expression vector. Expressed ECE was inhibited by phosphoramidon, but not by thiorphan. It also cleaved big ET-1 efficiently but not big ET-2 or big ET-3. Northern blot analysis revealed that ECE messenger RNA is widely expressed in many tissues.

Gene-Chemical Interaction Annotations    Click to see Annotation Detail View
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
phosphoramidon decreases activityISOEce1 (Rattus norvegicus)728596; 728596phosphoramidon decreases activity of recombinant Ece1 protein in COS cellsRGD 
phosphoramidon decreases activityEXP 728596phosphoramidon decreases activity of recombinant Ece1 protein in COS cellsRGD 

Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
protein processing  IDA 728596 RGD 

Objects Annotated

Genes (Rattus norvegicus)
Ece1  (endothelin converting enzyme 1)

Genes (Mus musculus)
Ece1  (endothelin converting enzyme 1)

Genes (Homo sapiens)
ECE1  (endothelin converting enzyme 1)


Additional Information