RGD Reference Report - Molecular cloning of a cDNA for alpha-subunit of rat liver electron transfer flavoprotein. - Rat Genome Database

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Molecular cloning of a cDNA for alpha-subunit of rat liver electron transfer flavoprotein.

Authors: Shinzawa, K  Inagaki, T  Ohishi, N  Ichihara, C  Tsukagoshi, N  Udaka, S  Yagi, K 
Citation: Shinzawa K, etal., Biochem Biophys Res Commun 1988 Aug 30;155(1):300-4.
RGD ID: 728564
Pubmed: PMID:3415685   (View Abstract at PubMed)

Two cDNA clones for the alpha-subunit of rat liver electron transfer flavoprotein were isolated and their nucleotide sequences were determined. The longer cDNA contained a protein-coding region of 900 nucleotides and 3'-noncoding region of 335 nucleotides. The identity of the clone was confirmed by matching the amino acid sequence predicted from the cDNA with the sequence of one of the lysyl endopeptidase-digested peptides from the purified alpha-subunit. The molecular weight of the protein calculated from the protein-coding nucleotides was approx. 3,000 daltons smaller than that of the precursor, suggesting that the cDNA was not of full length. The derived amino acid composition fairly agreed with the chemically determined amino acid composition of the purified alpha-subunit, indicating that the protein-coding region contains most of the mature alpha-subunit.

Gene Ontology Annotations    Click to see Annotation Detail View

Molecular Function
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
electron transfer activity  NAS 728564 RGD 

Objects Annotated

Genes (Rattus norvegicus)
Etfa  (electron transfer flavoprotein subunit alpha)


Additional Information